Weighted gene co-expression network analysis of hub genes in lung adenocarcinoma.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2021-04-12 eCollection Date: 2021-01-01 DOI:10.1177/11769343211009898

Xuan Luo, Lei Feng, WenBo Xu, XueJing Bai, MengNa Wu

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引用次数: 10

Abstract

Lung adenocarcinoma (LUAD) is a tumor with high incidence. This study aimed to identify the central genes of LUAD. LUAD were analyzed by weighted gene co-expression network (WGCNA), and differentially expressed genes (DEGs) were identified. Samples were obtained from The Cancer Genome Atlas (TCGA) and Genotype Tissue Expression (GTEx) databases and included 515 LUAD samples and 347 normal samples. The WGCNA algorithm generated a total of 10 modules. The top 2 modules (MEturquoise and MEblue) with the highest correlation to LUAD were selected. Ten Hub genes (IL6, CDH1, PECAM1, SPP1, THBS1, HGF, SNCA, CDH5, CAV1, and DLC1) were screened in the intersecting genes of DEGs and WGCNA (MEturquoise and MEblue). Only SPP1 was correlated with LUAD poor survival, indicating that SPP1 may be a key Hub gene for LUAD. The competing endogenous RNA (ceRNA) network was constructed to analyze the regulatory relationship of Hub genes, and SPP1 may be directly regulated by 4 microRNAs (miRNAs) and indirectly regulated by 49 long noncoding RNAs (lncRNAs).

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肺腺癌中心基因的加权基因共表达网络分析。

肺腺癌(LUAD)是一种高发的肿瘤。本研究旨在确定LUAD的中心基因。采用加权基因共表达网络(WGCNA)对LUAD进行分析，鉴定差异表达基因(deg)。样本来自癌症基因组图谱(TCGA)和基因型组织表达(GTEx)数据库，包括515例LUAD样本和347例正常样本。WGCNA算法共生成10个模块。选择与LUAD相关性最高的前2个模块(MEturquoise和MEblue)。在DEGs和WGCNA (MEturquoise和MEblue)的交叉基因中筛选出10个Hub基因(IL6、CDH1、PECAM1、SPP1、THBS1、HGF、SNCA、CDH5、CAV1和dcl1)。只有SPP1与LUAD不良生存相关，表明SPP1可能是LUAD的关键枢纽基因。构建竞争内源RNA (ceRNA)网络，分析Hub基因的调控关系，SPP1可能受到4种microRNAs (miRNAs)的直接调控，49种长链非编码RNA (lncRNAs)的间接调控。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.