Prevalence of feline leukaemia virus and feline immunodeficiency virus in domestic cats in Ireland.

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Acta veterinaria Hungarica Pub Date : 2021-01-16 DOI:10.1556/004.2020.00056

Anna Szilasi, Lilla Dénes, Eszter Krikó, Caoimhe Murray, Míra Mándoki, Gyula Balka

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引用次数: 5

Abstract

Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are retroviruses affecting felid species worldwide. A study was performed over a period of 5 months in Ireland with the aim to get an updated and more realistic prevalence of these retroviruses. A total of 183 EDTA-anticoagulated whole-blood samples were collected from cats distributed between 10 clinics. The samples were tested using both point-of-care enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Basic clinical data and vaccination history were also recorded for the sampled cats. The results of ELISA tests showed a prevalence of 10.4 and 3.3% for FIV and FeLV, respectively, and an apparent prevalence of 9.3% for FIV and 11.6% for FeLV with PCR. Phylogenetic analysis of the partial polymerase (pol) gene sequences obtained from 8 FIV-positive strains showed that all but one of the Irish strains belonged to FIV subtype A, and one to subtype B. The overall mean genetic similarity between the analysed strains was 91.15%.

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猫白血病病毒和猫免疫缺陷病毒在爱尔兰家猫中的流行。

猫免疫缺陷病毒(FIV)和猫白血病病毒(FeLV)是影响全世界猫科动物物种的逆转录病毒。在爱尔兰进行了为期5个月的研究，目的是获得这些逆转录病毒的最新和更现实的流行率。从分布在10个诊所的猫身上收集了183份edta抗凝全血样本。使用即时护理酶联免疫吸附试验(ELISA)和聚合酶链反应(PCR)对样品进行检测。还记录了取样猫的基本临床资料和疫苗接种史。ELISA检测结果显示FIV和FeLV的患病率分别为10.4%和3.3%，PCR检测结果显示FIV和FeLV的表观患病率分别为9.3%和11.6%。8株FIV阳性株的部分聚合酶(pol)基因序列的系统发育分析表明，爱尔兰株除1株外均属于FIV A亚型，1株属于b亚型，总体平均遗传相似性为91.15%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta veterinaria Hungarica 农林科学-兽医学

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： Acta Veterinaria Hungarica publishes original research papers presenting new scientific results of international interest, and to a limited extent also review articles and clinical case reports, on veterinary physiology (physiological chemistry and metabolism), veterinary microbiology (bacteriology, virology, immunology, molecular biology), on the infectious diseases of domestic animals, on veterinary parasitology, pathology, clinical veterinary science and reproduction.