Transcriptome Analysis of Pseudomonas aeruginosa Biofilm Following the Exposure to Malaysian Stingless Bee Honey.

Q2 Biochemistry, Genetics and Molecular Biology
Advances and Applications in Bioinformatics and Chemistry Pub Date : 2021-01-14 eCollection Date: 2021-01-01 DOI:10.2147/AABC.S292143
Nesrin Seder, Mohd Hilmi Abu Bakar, Walid Salem Abu Rayyan
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引用次数: 5

Abstract

Introduction: Malaysian stingless bee honey (Trigona) has been aroused as a potential antimicrobial compound with antibiofilm activity. The capability of the gram-negative bacillus P. aeruginosa to sustain a fatal infection is encoded in the bacterium genome.

Methods: In the current study, a transcriptome investigation was performed to explore the mechanism underlying the biofilm dispersal of P. aeruginosa after the exposure to Trigona honey.

Results: Microarray analysis of the Pseudomonas biofilm treated by 20% Trigona honey has revealed a down-regulation of 3478 genes among the 6085 screened genes. Specifically, around 13.5% of the down-regulated genes were biofilm-associated genes. The mapping of the biofilm-associated pathways has shown an ultimate decrease in the expression levels of the D-GMP signaling pathway and diguanylate cyclases (DGCs) genes responsible for c-di-GMP formation.

Conclusion: We predominantly report the lowering of c-di-GMP through the down-regulation of DGC genes as the main mechanism of biofilm inhibition by Trigona honey.

Abstract Image

Abstract Image

Abstract Image

马来西亚无刺蜂蜂蜜暴露后铜绿假单胞菌生物膜转录组分析。
摘要:马来西亚无刺蜂蜂蜜(Trigona)作为一种具有抗生物膜活性的潜在抗菌化合物已被人们所关注。革兰氏阴性杆菌铜绿假单胞菌维持致命感染的能力被编码在细菌基因组中。方法:本研究通过转录组研究,探讨假铜绿假单胞菌接触Trigona蜂蜜后生物膜扩散的机制。结果:对20% Trigona蜂蜜处理的假单胞菌生物膜进行微阵列分析,发现6085个筛选基因中有3478个基因下调。具体来说,大约13.5%的下调基因是生物膜相关基因。生物膜相关途径的图谱显示,D-GMP信号通路和负责c-di-GMP形成的二胍酸环化酶(DGCs)基因的表达水平最终下降。结论:我们主要报道了Trigona蜂蜜抑制生物膜的主要机制是通过下调DGC基因来降低c-di-GMP。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advances and Applications in Bioinformatics and Chemistry
Advances and Applications in Bioinformatics and Chemistry Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
6.50
自引率
0.00%
发文量
7
审稿时长
16 weeks
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