Effects of Inonotus obliquus Polysaccharides on Proliferation, Invasion, Migration, and Apoptosis of Osteosarcoma Cells.

IF 2.6 4区 医学 Q3 CELL BIOLOGY
Analytical Cellular Pathology Pub Date : 2020-11-17 eCollection Date: 2020-01-01 DOI:10.1155/2020/4282036
Baohui Su, Xuezhi Yan, Yuezhong Li, Junshan Zhang, Xiaoyan Xia
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引用次数: 7

Abstract

Objectives: To observe the effect of Inonotus obliquus polysaccharide (IOP) on the proliferation, invasion, migration, and apoptosis of osteosarcoma cells and to elucidate its underlying molecular mechanism.

Methods: IOP was extracted from Inonotus obliquus, human osteosarcoma MG-63 cells and U2OS cells were cultured in vitro, and the effects of IOP on the proliferation, migration, invasion, and apoptosis of MG-63 cells and U2OS cells were determined by CCK-8 assays, cell scratch assays, transwell assays, and flow cytometry, respectively. Western blot was used to detect the expression of related proteins in the Akt/mTOR and NF-κB signaling pathways.

Results: Compared with the control group, MG-63 cells and U2OS cells treated with IOP of 80 μg/ml, 160 μg/ml, and 320 μ g/ml in the experimental group had significantly lower proliferation activity, decreased migration and invasion ability, and increased apoptosis rate (P < 0.05). Furthermore, IOP could significantly inhibit the activation of the Akt/mTOR and NF-κB signaling pathway (P < 0.05).

Conclusion: IOP can regulate the proliferation, migration, invasion, and apoptosis of osteosarcoma cells by inhibiting the activation of the Akt/mTOR signaling pathway. It has antitumor activity on osteosarcoma and has the potential of clinical application in osteosarcoma treatment.

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斜凤尾多糖对骨肉瘤细胞增殖、侵袭、迁移和凋亡的影响。
目的:观察斜棘多糖(IOP)对骨肉瘤细胞增殖、侵袭、迁移和凋亡的影响,并探讨其分子机制。方法:从斜腹肌提取IOP,体外培养人骨肉瘤MG-63细胞和U2OS细胞,分别采用CCK-8法、细胞划痕法、transwell法和流式细胞术检测IOP对MG-63细胞和U2OS细胞增殖、迁移、侵袭和凋亡的影响。Western blot检测Akt/mTOR和NF-κB信号通路中相关蛋白的表达。结果:与对照组相比,80、160、320 μg/ml IOP处理组MG-63细胞和U2OS细胞的增殖活性显著降低,迁移和侵袭能力下降,凋亡率显著升高(P < 0.05)。此外,IOP可显著抑制Akt/mTOR和NF-κB信号通路的激活(P < 0.05)。结论:IOP可通过抑制Akt/mTOR信号通路的激活,调控骨肉瘤细胞的增殖、迁移、侵袭和凋亡。对骨肉瘤具有抗肿瘤活性,在骨肉瘤治疗中具有临床应用潜力。
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来源期刊
Analytical Cellular Pathology
Analytical Cellular Pathology ONCOLOGY-CELL BIOLOGY
CiteScore
4.90
自引率
3.10%
发文量
70
审稿时长
16 weeks
期刊介绍: Analytical Cellular Pathology is a peer-reviewed, Open Access journal that provides a forum for scientists, medical practitioners and pathologists working in the area of cellular pathology. The journal publishes original research articles, review articles, and clinical studies related to cytology, carcinogenesis, cell receptors, biomarkers, diagnostic pathology, immunopathology, and hematology.
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