Analysis of Anopheles messeae s.l. intron gene polymorphism associated with imidacloprid resistance

IF 1.4 3区农林科学 Q2 ENTOMOLOGY

Journal of Vector Ecology Pub Date : 2020-11-18 DOI:10.1111/jvec.12393

Oleg V. Vaulin, Dmitry. A. Karagodin, Tatiana A. Novgorodova, Viktor V. Glupov

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引用次数: 1

Abstract

Due to their high solubility and stability, neonicotinoid insecticides are able to accumulate in water bodies, affecting aquatic organisms. The aims of this study were to evaluate resistance (LC₅₀) of Anopheles messeae s.l. (Anopheles messeae and An. daciae) to the neonicotinoid imidacloprid and to search for genetic markers associated with insecticide resistance. The LC₅₀ values of these species in the collections during 2017 and 2018 were indistinguishable and were in the range of 0.027–0.051 mg/l. In general, the LC₅₀ values of the mosquitoes were comparable with values of other mosquito species of the Anopheles and Culex genera. Gene polymorphisms of the variations in intron lengths and the presence of restriction sites in introns that were potentially associated with the metabolism of insecticides were studied. Polymorphisms of the studied genes in the pair of closely related species considered overlapped, but allele frequencies were different. Part of the genetic variants arose due to insertions of repetitive elements of the genome. Two variants of the cytochrome P450 gene Cyp6AG1 in An. daciae were associated with increased resistance to imidacloprid. Possible side effects of selection on insecticide resistance in blood-sucking mosquitoes are discussed.

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讯息按蚊吡虫啉抗性相关内含子基因多态性分析

新烟碱类杀虫剂由于其高溶解度和稳定性，可在水体中积累，影响水生生物。本研究的目的是评价埃及按蚊(Anopheles messseae)和埃及按蚊(Anopheles messseae)的抗药性(LC50)。达契亚科(Daciae)对新烟碱类吡虫啉(imidacloprid)的抗性，并寻找与抗药性相关的遗传标记。2017年和2018年的LC50值在0.027 ~ 0.051 mg/l之间，无明显差异。总体而言，该蚊的LC50值与按蚊属和库蚊属其他蚊种的LC50值相当。研究了内含子长度变异的基因多态性以及内含子中可能与杀虫剂代谢有关的限制性内切位点的存在。被研究的基因在近缘种对中的多态性被认为是重叠的，但等位基因频率不同。部分遗传变异是由于基因组重复元素的插入而产生的。细胞色素P450基因Cyp6AG1的两种变异。Daciae与吡虫啉抗性增强有关。讨论了选择对吸血蚊抗药性可能产生的副作用。

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来源期刊

Journal of Vector Ecology 生物-昆虫学

CiteScore

2.60

自引率

5.90%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Vector Ecology is an international journal published by the Society for Vector Ecology. It is concerned with all aspects of the biology, ecology, and control of arthropod and vertebrate vectors and the interrelationships between the vectors and the agents of disease that they transmit. The journal publishes original research articles and scientific notes, as well as comprehensive reviews of vector biology based on presentations at Society meetings. All papers are reviewed by at least two qualified scientists who recommend their suitability for publication. Acceptance of manuscripts is based on their scientific merit and is the final decision of the editor, but these decisions may be appealed to the editorial board. The journal began publishing in 1974 and now publishes on-line only.