Nanoscale mechanical properties of chitosan hydrogels as revealed by AFM.

IF 4.4 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Progress in Biomaterials Pub Date : 2020-12-01 Epub Date: 2020-11-06 DOI:10.1007/s40204-020-00141-4
A Ben Bouali, A Montembault, L David, Y Von Boxberg, M Viallon, B Hamdi, F Nothias, R Fodil, S Féréol
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引用次数: 13

Abstract

In the context of tissue engineering, chitosan hydrogels are attractive biomaterials because they represent a family of natural polymers exhibiting several suitable features (cytocompatibility, bioresorbability, wound healing, bacteriostatic and fungistatic properties, structural similarity with glycosaminoglycans), and tunable mechanical properties. Optimizing the design of these biomaterials requires fine knowledge of its physical characteristics prior to assessment of the cell-biomaterial interactions. In this work, using atomic force microscopy (AFM), we report a characterization of mechanical and topographical properties at the submicron range of chitosan hydrogels, depending on physico-chemical parameters such as their polymer concentration (1.5%, 2.5% and 3.5%), their degree of acetylation (4% and 38.5%), and the conditions of the gelation process. Well-known polyacrylamide gels were used to validate the methodology approach for the determination and analysis of elastic modulus (i.e., Young's modulus) distribution at the gel surface. We present elastic modulus distribution and topographical and stiffness maps for different chitosan hydrogels. For each chitosan hydrogel formulation, AFM analyses reveal a specific asymmetric elastic modulus distribution that constitutes a useful hallmark for chitosan hydrogel characterization. Our results regarding the local mechanical properties and the topography of chitosan hydrogels initiate new possibilities for an interpretation of the behavior of cells in contact with such soft materials.

原子力显微镜研究壳聚糖水凝胶的纳米力学性能。
在组织工程的背景下,壳聚糖水凝胶是一种有吸引力的生物材料,因为它们代表了一系列天然聚合物,具有几种合适的特性(细胞相容性、生物可吸收性、伤口愈合性、抑菌和抑菌性能、与糖胺聚糖的结构相似性)和可调的机械性能。优化这些生物材料的设计需要在评估细胞-生物材料相互作用之前对其物理特性有很好的了解。在这项工作中,我们使用原子力显微镜(AFM)报道了壳聚糖水凝胶在亚微米范围内的力学和地形特性,这取决于它们的聚合物浓度(1.5%,2.5%和3.5%),它们的乙酰化程度(4%和38.5%)以及凝胶化过程的条件等物理化学参数。使用知名的聚丙烯酰胺凝胶来验证测定和分析凝胶表面弹性模量(即杨氏模量)分布的方法学方法。我们给出了不同壳聚糖水凝胶的弹性模量分布、地形和刚度图。对于每种壳聚糖水凝胶配方,AFM分析揭示了特定的不对称弹性模量分布,这构成了壳聚糖水凝胶表征的有用标志。我们关于壳聚糖水凝胶的局部力学性能和形貌的研究结果为解释细胞与这种软材料接触时的行为提供了新的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Progress in Biomaterials
Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
9.60
自引率
4.10%
发文量
35
期刊介绍: Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.
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