Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2.

IF 2.6 4区医学 Q3 CELL BIOLOGY

Analytical Cellular Pathology Pub Date : 2020-10-15 eCollection Date: 2020-01-01 DOI:10.1155/2020/3731953

Leilei Xu, Qin Zhang, Changhua Li, Fu Hua, Xiaoping Liu

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Abstract

The application of ultrasound and microbubbles (USMB-) mediated microRNA (miR) is a promising approach of gene delivery for cancer treatment. We aimed to discuss the effects of USMB-miR-505 on cervical cancer (CC) development. miR-505 mediated by USMB was prepared. The effect of miR-505 on its transfection efficiency and the effect of miR-505 on HeLa cell proliferation, cell cycle, apoptosis, migration, and invasion were studied. The target gene of miR-505 was predicted, and its expression in CC was detected. The effect of the target gene on HeLa cells was further verified. USMB-miR-505 showed a higher transfection efficiency than miR-505 alone. The inhibitory effect of miR-505 mediated by USMB on HeLa cells was better than miR-505. miR-505 targeted AKT2, which was upregulated in CC. Overexpression of AKT2 reversed the inhibitory effect of USMB-miR-505 on HeLa cell malignant behaviors. Overall, we highlighted that USMB-miR-505 inhibited HeLa cell malignant behaviors by targeting AKT2.

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超声微泡介导的microRNA-505通过AKT2调控宫颈癌细胞生长。

应用超声和微泡(USMB-)介导的microRNA (miR)是一种很有前途的癌症基因传递方法。我们旨在讨论USMB-miR-505对宫颈癌(CC)发展的影响。制备USMB介导的miR-505。研究miR-505对其转染效率的影响，以及miR-505对HeLa细胞增殖、细胞周期、凋亡、迁移和侵袭的影响。预测miR-505的靶基因，并检测其在CC中的表达。进一步验证了靶基因对HeLa细胞的作用。USMB-miR-505的转染效率高于单独转染miR-505。USMB介导的miR-505对HeLa细胞的抑制作用优于miR-505。miR-505靶向在CC中上调的AKT2, AKT2过表达逆转了USMB-miR-505对HeLa细胞恶性行为的抑制作用。总之，我们强调了USMB-miR-505通过靶向AKT2抑制HeLa细胞的恶性行为。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical Cellular Pathology ONCOLOGY-CELL BIOLOGY

CiteScore

4.90

自引率

3.10%

发文量

审稿时长

16 weeks

期刊介绍： Analytical Cellular Pathology is a peer-reviewed, Open Access journal that provides a forum for scientists, medical practitioners and pathologists working in the area of cellular pathology. The journal publishes original research articles, review articles, and clinical studies related to cytology, carcinogenesis, cell receptors, biomarkers, diagnostic pathology, immunopathology, and hematology.