Isolation, identification and chemical inactivation of infectious laryngotracheitis virus for use as a vaccine candidate.

IF 1.1 4区医学 Q4 VIROLOGY

Acta virologica Pub Date : 2021-01-01 DOI:10.4149/av_2021_101

Mohammad Majid Ebrahimi, Shahla Shahsavandi, Ali Reza Yousefi, Nikdokht Ebrahimi

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引用次数: 1

Abstract

Infectious laryngotracheitis (ILT) is a poultry respiratory disease associated with considerable mortality in chicken and decreasing egg production. Vaccination along with biosecurity measures are considered as the main strategy for ILT control. This study was aimed to evaluate the potency of an inactive ILT vaccine candidate derived from a local ILTV isolate. The isolated virus was characterized and treated with various chemicals and their concentrations. The virus infectivity was entirely abolished by treatment of 3 mM binary ethylene imine following 16 h incubation. The immune response of inactivated ILTV suspension with adjuvans was evaluated in both SPF chickens (experiment-I) and Hyline pullets (experiment-II). Efficacy of the inactivated and live ILT vaccines combination was compared. The results of experiment-I showed that the inactivated antigen induced specific antibody titers against ILTV. In experiment-II, despite the increase in serum antibody level administration of the inactivated antigen alone did not offer sufficient protection. The full protection was found in chickens that received the combination regimen. We conclude that simultaneous administration of the inactivated and live ILT vaccines was efficient for induction of immunity against ILTV. Keywords: infectious laryngotracheitis virus; vaccine; inactivation; immune response.

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传染性喉气管炎候选疫苗病毒的分离、鉴定和化学灭活。

传染性喉气管炎(ILT)是一种家禽呼吸道疾病，与鸡的大量死亡率和产蛋率下降有关。疫苗接种和生物安全措施被认为是控制ILT的主要策略。本研究旨在评估从当地ILTV分离物中提取的非活性ILT候选疫苗的效力。对分离的病毒进行了表征，并用各种化学物质及其浓度进行了处理。3 mM二乙烯亚胺处理16 h后，病毒的传染性完全消失。在SPF鸡(实验- 1)和Hyline鸡(实验- 2)中评价了含佐剂的灭活ILTV悬浮液的免疫应答。比较灭活疫苗和ILT活疫苗联合使用的疗效。实验1的结果表明，灭活抗原诱导了针对ILTV的特异性抗体滴度。在实验二中，尽管血清抗体水平升高，单独给予灭活抗原并不能提供足够的保护。在接受联合治疗的鸡中发现了充分的保护作用。我们的结论是，同时给药灭活疫苗和活ILT疫苗是有效的诱导免疫对抗ILTV。关键词:传染性喉气管炎病毒;疫苗;失活;免疫反应。

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来源期刊

Acta virologica 医学-病毒学

CiteScore

3.10

自引率

11.80%

发文量

审稿时长

>12 weeks

期刊介绍： Acta virologica is an international journal of predominantly molecular and cellular virology. Acta virologica aims to publish papers reporting original results of fundamental and applied research mainly on human, animal and plant viruses at cellular and molecular level. As a matter of tradition, also rickettsiae are included. Areas of interest are virus structure and morphology, molecular biology of virus-cell interactions, molecular genetics of viruses, pathogenesis of viral diseases, viral immunology, vaccines, antiviral drugs and viral diagnostics.