Extraction, GC-MS Evaluation and Anti-epileptic Potential of Seeds Ethanolic Extract of Putranjiva roxburghii Wall.

Q3 Psychology

Central nervous system agents in medicinal chemistry Pub Date : 2020-01-01 DOI:10.2174/1871524920999201027125743

Chandra Kala, Syed S Imam, Mohamad Taleuzzaman, Sadaf J Gilani, Syed S Ali, Iqra Rahat, Adil Ahmad, Najam A Khan

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引用次数: 1

Abstract

Background: Putranjiva roxburghii Wall is traditionally known to cure many pathological conditions including epilepsy.

Objective: The present study is aimed at determining bioactive compounds in ethanolic extract of Putranjiva roxburghii test extract (PRTE) seeds by GCMS analysis and to assess its antiepileptic potential using various experimental models of epilepsy.

Methods: The ethanolic extract of seeds of Putranjiva roxburghii was subjected to GC-MS analysis to detect the bioactive phytoconstituents. Acute oral toxicity of the extract was performed using OCED guideline 420. Pentylenetetrazol (PTZ) kindling model of epilepsy and Maximal electroshock epilepsy (MES) model of epilepsy were used to determine anti-epileptic potential.

Results: The GC-MS analysis of the extract revealed the presence of 20 phytoconstituents. The major phytoconstituents included n-Propyl heptyl ether (25.25%), 5-Ethyl hydantoin (8%), octadec- 9-enoic acid (16.25%) and 1, 2-Benzene dicarboxylic acid (11.86%). The PRTE (50 mg/kg and 100 mg/kg) afforded a significant and dose-dependent protection against PTZ-induced kindling epilepsy and MES induced epilepsy (p<0.001 and p<0.01).

Conclusion: Based on the above findings, it is evident that Putranjiva roxburghii seeds contain biologically active compounds. It can also be concluded that the extract possesses anti-epileptic potential.

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刺梨籽醇提物的提取、GC-MS评价及抗癫痫作用。

背景:传统上认为黄刺藤可以治疗包括癫痫在内的许多病理性疾病。目的:采用气相色谱法测定黄刺荆芥提取物(PRTE)种子乙醇提取物中的活性成分，并通过多种癫痫实验模型评价其抗癫痫作用。方法:采用气相色谱-质谱联用技术，对刺梨籽醇提物进行生物活性成分检测。根据OCED指南420对提取物进行急性口服毒性试验。采用戊四唑(PTZ)点燃癫痫模型和最大电休克癫痫(MES)模型测定抗癫痫电位。结果:经GC-MS分析，提取液中含有20种植物成分。主要植物成分为正丙基庚醚(25.25%)、5-乙基海因(8%)、八烷基- 9-烯酸(16.25%)和1,2 -苯二甲酸(11.86%)。PRTE (50 mg/kg和100 mg/kg)对ptz诱导的点燃性癫痫和MES诱导的癫痫具有显著且剂量依赖性的保护作用(p)。结论:上述结果表明，刺槐种子含有生物活性物质。该提取物具有抗癫痫作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Central nervous system agents in medicinal chemistry Psychology-Neuropsychology and Physiological Psychology

CiteScore

2.10

自引率

0.00%

发文量

期刊介绍： Central Nervous System Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new central nervous system agents. Containing a series of timely in-depth reviews written by leaders in the field covering a range of current topics, Central Nervous System Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in the field.