Allison H Rietze, Yvette P Conley, Dianxu Ren, Cindy M Anderson, James M Roberts, Arun Jeyabalan, Carl A Hubel, Mandy J Schmella
{"title":"DNA Methylation of Endoglin Pathway Genes in Pregnant Women With and Without Preeclampsia.","authors":"Allison H Rietze, Yvette P Conley, Dianxu Ren, Cindy M Anderson, James M Roberts, Arun Jeyabalan, Carl A Hubel, Mandy J Schmella","doi":"10.1177/2516865720959682","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>We compared blood-based DNA methylation levels of endoglin (<i>ENG</i>) and transforming growth factor beta receptor 2 (<i>TGFβR2</i>) gene promoter regions between women with clinically-overt preeclampsia and women with uncomplicated, normotensive pregnancies.</p><p><strong>Methods: </strong>We used EpiTect Methyl II PCR Assays to evaluate DNA methylation of CpG islands located in promoter regions of <i>ENG</i> (CpG Island 114642) and <i>TGFβR2</i> (CpG Island 110111). Preeclampsia was diagnosed based on blood pressure, protein, and uric acid criteria. N = 21 nulliparous preeclampsia case participants were 1:1 frequency matched to N = 21 nulliparous normotensive control participants on gestational age at sample collection (±2 weeks), smoking status, and labor status at sample collection. Methylation values were compared between case and control participant groups [(<i>ENG</i> subset: n = 20 (9 cases, 11 controls); <i>TGFβR2</i> subset: n = 28 (15 cases, 13 controls)].</p><p><strong>Results: </strong>The majority of the preeclampsia cases delivered at ⩾34 weeks' gestation (83%). Average methylation levels for <i>ENG</i> ([M ± (SD)]; Case Participant Group = 6.54% ± 4.57 versus Control Participant group = 4.81% ± 5.08; <i>P</i> = .102) and <i>TGFβR2</i> (Case Participant Group = 1.50% ± 1.37 vs Control Participant Group = 1.70% ± 1.40; <i>P</i> = .695) promoter CpG islands did not differ significantly between the participant groups. Removal of 2 extreme outliers in the <i>ENG</i> analytic subset revealed a trend between levels of <i>ENG</i> methylation and pregnancy outcome (Case Participant Group = 5.17% ± 2.16 vs Control Participant Group = 3.36% ± 1.73; <i>P</i> = .062).</p><p><strong>Conclusion: </strong>Additional epigenetic studies that include larger sample sizes, investigate preeclampsia subtypes, and capture methylation status of CpG island shores and shelves are needed to further inform us of the potential role that <i>ENG</i> and <i>TGFβR2</i> DNA methylation plays in preeclampsia pathophysiology.</p>","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":" ","pages":"2516865720959682"},"PeriodicalIF":4.3000,"publicationDate":"2020-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/2516865720959682","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/2516865720959682","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: We compared blood-based DNA methylation levels of endoglin (ENG) and transforming growth factor beta receptor 2 (TGFβR2) gene promoter regions between women with clinically-overt preeclampsia and women with uncomplicated, normotensive pregnancies.
Methods: We used EpiTect Methyl II PCR Assays to evaluate DNA methylation of CpG islands located in promoter regions of ENG (CpG Island 114642) and TGFβR2 (CpG Island 110111). Preeclampsia was diagnosed based on blood pressure, protein, and uric acid criteria. N = 21 nulliparous preeclampsia case participants were 1:1 frequency matched to N = 21 nulliparous normotensive control participants on gestational age at sample collection (±2 weeks), smoking status, and labor status at sample collection. Methylation values were compared between case and control participant groups [(ENG subset: n = 20 (9 cases, 11 controls); TGFβR2 subset: n = 28 (15 cases, 13 controls)].
Results: The majority of the preeclampsia cases delivered at ⩾34 weeks' gestation (83%). Average methylation levels for ENG ([M ± (SD)]; Case Participant Group = 6.54% ± 4.57 versus Control Participant group = 4.81% ± 5.08; P = .102) and TGFβR2 (Case Participant Group = 1.50% ± 1.37 vs Control Participant Group = 1.70% ± 1.40; P = .695) promoter CpG islands did not differ significantly between the participant groups. Removal of 2 extreme outliers in the ENG analytic subset revealed a trend between levels of ENG methylation and pregnancy outcome (Case Participant Group = 5.17% ± 2.16 vs Control Participant Group = 3.36% ± 1.73; P = .062).
Conclusion: Additional epigenetic studies that include larger sample sizes, investigate preeclampsia subtypes, and capture methylation status of CpG island shores and shelves are needed to further inform us of the potential role that ENG and TGFβR2 DNA methylation plays in preeclampsia pathophysiology.
期刊介绍:
ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric.
Indexed/Abstracted:
Web of Science SCIE
Scopus
CAS
INSPEC
Portico