Expression of the matrix metalloproteinases MMP-2 and MMP-9 and their inhibitors TIMP-1 and TIMP-2 in systemic lupus erythematosus patients.

4区医学 Q3 Medicine

Netherlands Journal of Medicine Pub Date : 2020-09-01

H J Vira, V D Pradhan, V D Umare, A K Chaudhary, A G Rajadhyksha, M Y Nadkar, K Ghosh, A H Nadkarni

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引用次数: 0

Abstract

Background: The study aimed to look at alterations in expression of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) and their potential use as biomarkers in the pathogensis of SLE.

Methods: SLE patients (n = 41) and healthy controls (n = 50) were recruited. Quantitative RT-PCR/ELISA assays were performed for expression of MMP and TIMP mRNA in whole blood and PBMC; and corresponding serum protein levels. Intracellular levels of MMP-2 and MMP-9 proteins were analysed by flow cytometry.

Results: Based on SLEDAI scores patients were grouped into active (SLEDAI ≥ 10) and inactive cases (SLEDAI < 10). In active cases, MMP-2 expression significantly increased and TIMP-2 expression was decreased (p < 0.0001) both at serum secretion (p = 0.0003) and mRNA (p < 0.0001) levels as compared to inactive cases. MMP-9 and TIMP-1 showed significantly reduced serum secretion and mRNA expression (p < 0.0001) in active cases as compared to inactive cases. Intracellular concentration of MMP-9 was reported to be higher in neutrophils, while MMP-2 was mainly found in lymphocytes of SLE patients as compared to controls. MMP/TIMP ratio profile was altered as SLE disease progresses.

Interpretation & conclusions: Findings suggest disturbed MMP and TIMP levels have a role in the pathogenesis of SLE.

本刊更多论文

基质金属蛋白酶MMP-2、MMP-9及其抑制剂TIMP-1、TIMP-2在系统性红斑狼疮患者中的表达

背景:该研究旨在观察基质金属蛋白酶(MMPs)及其组织抑制剂(TIMPs)的表达变化及其在SLE发病机制中作为生物标志物的潜在用途。方法:招募SLE患者(n = 41)和健康对照组(n = 50)。采用定量RT-PCR/ELISA法检测全血和PBMC中MMP、TIMP mRNA的表达;以及相应的血清蛋白水平。流式细胞术检测细胞内MMP-2和MMP-9蛋白水平。结果:根据SLEDAI评分将患者分为活动期(SLEDAI≥10)和非活动期(SLEDAI < 10)。活性组血清分泌量(p = 0.0003)和mRNA水平(p < 0.0001)均显著高于活性组(p < 0.0001)， TIMP-2表达显著低于活性组(p < 0.0001)。活性组血清MMP-9和TIMP-1的分泌量和mRNA表达量显著低于非活性组(p < 0.0001)。与对照组相比，MMP-9在中性粒细胞中的细胞内浓度较高，而MMP-2主要存在于SLE患者的淋巴细胞中。随着SLE疾病的进展，MMP/TIMP比值谱发生改变。解释和结论:研究结果表明紊乱的MMP和TIMP水平在SLE的发病机制中起作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Netherlands Journal of Medicine 医学-医学：内科

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Netherlands Journal of Medicine publishes papers in all relevant fields of internal medicine. In addition to reports of original clinical and experimental studies, reviews on topics of interest or importance, case reports, book reviews and letters to the editor are welcomed.