Antibacterial Activity of Copper Nanoparticles-Chitosan Composite against Vibrio parahaemolyticus.

IF 0.9 4区农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocontrol science Pub Date : 2020-01-01 DOI:10.4265/bio.25.159

Ngoc-Yen Nguyen, Bui Nguyet An, Minh-Vien LE, Hoang A Hoang

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引用次数: 4

Abstract

The Early Mortality Syndrome (EMS) caused by Vibrio parahaemolyticus has recently resulted in a serious loss in shrimp farms in the Mekong delta, Vietnam. Here, antibacterial activity of copper nanoparticles-chitosan composite (CuCS) against V. parahaemolyticus was investigated. Copper nanoparticles were synthesized using L-ascorbic acid as a green reducing agent and chitosan as a biopolymer matrix and stabilizing agent. The physical properties of CuCS were evaluated. Next, antibacterial activity of 2.5, 5.0, 10.0 ppm CuCS against V. parahaemolyticus inoculated in a sterilized shrimp-pond water was examined. CuCS at 2.5 ppm eliminated 91.47% and 95.26% of V. parahaemolyticus after 2 and 4 h of exposure, respectively. Complete elimination was attained following 2 h of 5.0 ppm CuCS exposure. A complete elimination of V. parahaemolyticus in a real EMS-infected shrimp-pond water was also described. This study is the first to report the antibacterial activity of CuCS against V. parahaemolyticus, an important pathogen in shrimp industry in the Mekong delta, Vietnam.

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纳米铜-壳聚糖复合材料对副溶血性弧菌的抑菌活性研究。

由副溶血性弧菌引起的早期死亡综合征(EMS)最近在越南湄公河三角洲的对虾养殖场造成了严重损失。本文研究了纳米铜-壳聚糖复合材料(CuCS)对副溶血性弧菌的抗菌活性。以l -抗坏血酸为绿色还原剂，壳聚糖为生物聚合物基质和稳定剂，合成了铜纳米颗粒。对CuCS的物理性质进行了评价。其次，研究了2.5、5.0、10.0 ppm CuCS对经消毒的虾池水接种的副溶血性弧菌的抑菌活性。浓度为2.5 ppm的氯化碳在暴露2 h和4 h后，对副溶血性弧菌的去除率分别为91.47%和95.26%。在5.0 ppm的ccu暴露2小时后，完全消除。在一个真正的ems感染的虾塘水完全消除副溶血性弧菌也被描述。本研究首次报道了CuCS对越南湄公河三角洲对虾业重要病原菌副溶血性弧菌的抑菌活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biocontrol science BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

2.60

自引率

8.30%

发文量

审稿时长

>12 weeks

期刊介绍： The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.