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{"title":"Nuclease Degradation Analysis of DNA Nanostructures Using Gel Electrophoresis","authors":"Arun Richard Chandrasekaran, Ken Halvorsen","doi":"10.1002/cpnc.115","DOIUrl":null,"url":null,"abstract":"<p>Custom-built DNA nanostructures are now used in applications such as biosensing, molecular computation, biomolecular analysis, and drug delivery. While the functionality and biocompatibility of DNA makes DNA nanostructures useful in such applications, the field faces a challenge in making biostable DNA nanostructures. Being a natural material, DNA is most suited for biological applications, but is also easily degraded by nucleases. Several methods have been employed to study the nuclease degradation rates and enhancement of nuclease resistance. This protocol describes the use of gel electrophoresis to analyze the extent of nuclease degradation of DNA nanostructures and to report degradation times, kinetics of nuclease digestion, and evaluation of biostability enhancement factors. © 2020 Wiley Periodicals LLC.</p><p><b>Basic Protocol</b>: Timed analysis of nuclease degradation of DNA nanostructures</p><p><b>Support Protocol</b>: Calculating biostability enhancement factors</p>","PeriodicalId":10966,"journal":{"name":"Current Protocols in Nucleic Acid Chemistry","volume":"82 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpnc.115","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Nucleic Acid Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpnc.115","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Chemistry","Score":null,"Total":0}
引用次数: 11
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Abstract
Custom-built DNA nanostructures are now used in applications such as biosensing, molecular computation, biomolecular analysis, and drug delivery. While the functionality and biocompatibility of DNA makes DNA nanostructures useful in such applications, the field faces a challenge in making biostable DNA nanostructures. Being a natural material, DNA is most suited for biological applications, but is also easily degraded by nucleases. Several methods have been employed to study the nuclease degradation rates and enhancement of nuclease resistance. This protocol describes the use of gel electrophoresis to analyze the extent of nuclease degradation of DNA nanostructures and to report degradation times, kinetics of nuclease digestion, and evaluation of biostability enhancement factors. © 2020 Wiley Periodicals LLC.
Basic Protocol : Timed analysis of nuclease degradation of DNA nanostructures
Support Protocol : Calculating biostability enhancement factors
核酸酶降解DNA纳米结构的凝胶电泳分析
定制的DNA纳米结构现在应用于生物传感、分子计算、生物分子分析和药物输送等领域。虽然DNA的功能和生物相容性使得DNA纳米结构在这些应用中很有用，但该领域面临着制造生物稳定的DNA纳米结构的挑战。作为一种天然材料，DNA最适合生物应用，但也很容易被核酸酶降解。采用了几种方法来研究核酸酶的降解率和核酸酶抗性的增强。该方案描述了使用凝胶电泳来分析DNA纳米结构的核酸酶降解程度，并报告降解时间，核酸酶消化动力学和生物稳定性增强因子的评估。©2020 Wiley期刊公司基本方案:核酸酶降解DNA纳米结构的定时分析支持方案:计算生物稳定性增强因子
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