The stimulative function of long noncoding RNA CDKN2B-AS1 in osteosarcoma by targeting the microRNA-122/CCNG1 axis.

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Receptors and Signal Transduction Pub Date : 2022-02-01 Epub Date: 2020-12-06 DOI:10.1080/10799893.2020.1850784

Abulaiti Abula, Guliayixiamu Saimaiti, Xayimardan Maimaiti, Wumitijiang Wuqikun, Alimujiang Abulaiti, Peng Ren, Aihemaitijiang Yusufu

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引用次数: 5

Abstract

Osteosarcoma (OS), a prevalent aggressive malignancy in the bone, has limited therapeutic targets and diagnostic biomarkers. In the current investigation, RT-qPCR showed that CDKN2B-AS1 was enhanced in OS samples and cells. This research was set to examine the modulation of CDKN2B-AS1 in OS. The expression of CDKN2B-AS1 and downstream molecules was analyzed by RT-qPCR method. CCK8, EdU staining along with Transwell assays were applied to evaluate cell proliferation and invasion. Those in vitro investigations specified that silencing of CDKN2B-AS1 with shRNAs obviously impeded the proliferation and invasion of MG63 cells. To authenticate the relationships between CDKN2B-AS1 and microRNA-122-5p (miR-122-5p) or cyclin G1 (CCNG1) and miR-122-5p, we next employed luciferase reporter assay. We displayed that CDKN2B-AS1 repressed miR-122-5p to restore CCNG1 expression. All in all, our findings substantiated the indispensable function of CDKN2B-AS1 in OS progression and the possible molecular mechanism.

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靶向microRNA-122/CCNG1轴的长链非编码RNA CDKN2B-AS1在骨肉瘤中的刺激作用

骨肉瘤(OS)是一种常见的骨恶性肿瘤，其治疗靶点和诊断生物标志物有限。在本研究中，RT-qPCR显示CDKN2B-AS1在OS样本和细胞中增强。本研究旨在检测CDKN2B-AS1在OS中的调制。RT-qPCR法分析CDKN2B-AS1及其下游分子的表达。CCK8、EdU染色及Transwell法检测细胞增殖及侵袭情况。这些体外研究表明，用shrna沉默CDKN2B-AS1明显阻碍了MG63细胞的增殖和侵袭。为了验证CDKN2B-AS1与microRNA-122-5p (miR-122-5p)或细胞周期蛋白G1 (CCNG1)与miR-122-5p之间的关系，我们接下来采用荧光素酶报告基因检测。我们发现CDKN2B-AS1抑制miR-122-5p以恢复CCNG1的表达。总之，我们的研究结果证实了CDKN2B-AS1在OS进展中不可或缺的功能及其可能的分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Receptors and Signal Transduction 生物-生化与分子生物学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Receptors and Signal Tranduction is included in the following abstracting and indexing services: BIOBASE; Biochemistry and Biophysics Citation Index; Biological Abstracts; BIOSIS Full Coverage Shared; BIOSIS Previews; Biotechnology Abstracts; Current Contents/Life Sciences; Derwent Chimera; Derwent Drug File; EMBASE; EMBIOLOGY; Journal Citation Reports/ Science Edition; PubMed/MedLine; Science Citation Index; SciSearch; SCOPUS; SIIC.