Assessment of Problem-Solving Skills and Inhibitory Control in Mice Using Water Escape Detour Test

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2020-09-01 DOI:10.1002/cpmo.82

Grzegorz R. Juszczak, Anna Bobrowska

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引用次数: 1

Abstract

Despite the importance of emotional intelligence, its biological mechanism is still not well understood. For this reason, we have developed a rodent detour task which requires an animal to reach a highly desired object placed directly behind a transparent barrier that blocks the direct route to the target. This apparently simple task is highly dependent on the emotional control that is necessary to inhibit prepotent and counterproductive responses driven by the sight of a desired object. The water escape detour task designed for mice enables testing the ability to solve emotionally challenging problems, as well as identification of an impairment termed perseveration. Such a maladaptive reaction to a challenging situation is characterized by difficulty in terminating an unsuccessful response, leading to persistent repetition of inappropriate behavior. This issue is important because perseveration is associated with schizophrenia, drug abuse, and aging. © 2020 Wiley Periodicals LLC.

Basic Protocol: Water escape detour task

Support Protocol 1: Preparation of escape platform

Support Protocol 2: Preparation of the transparent barrier

Alternate Protocol: Water escape detour task for testing acute effects

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水逃逸绕道实验对小鼠问题解决能力和抑制控制的评价

尽管情商很重要，但其生物学机制仍未得到很好的理解。出于这个原因，我们开发了一个啮齿类动物绕路任务，要求动物到达一个高度期望的目标，这个目标被放置在一个透明的屏障后面，挡住了通往目标的直接路线。这个看似简单的任务高度依赖于情绪控制，而情绪控制是抑制由看到想要的物体而引起的强势反应和适得其反的反应所必需的。为老鼠设计的水逃逸绕路任务可以测试解决情感挑战问题的能力，以及识别被称为毅力的损伤。这种对具有挑战性的情况的不适应反应的特点是难以终止不成功的反应，导致不适当行为的持续重复。这个问题很重要，因为毅力与精神分裂症、药物滥用和衰老有关。©2020 Wiley期刊有限责任公司基本协议:水逃逸绕行任务支持协议1:准备逃逸平台支持协议2:准备透明屏障备用协议:测试急性效应的水逃逸绕行任务

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来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.