A Low-Cost Tebuconazole-Based Screening Test for Azole-Resistant Aspergillus fumigatus

Current Protocols in Microbiology Pub Date : 2020-08-28 DOI:10.1002/cpmc.112

Amelie P. Brackin, Jennifer M. G. Shelton, Alireza Abdolrasouli, Matthew C. Fisher, Thomas R. Sewell

引用次数: 4

Abstract

The global emergence of azole resistance in Aspergillus fumigatus is resulting in health and food security concerns. Rapid diagnostics and environmental surveillance methods are key to understanding the distribution and prevalence of azole resistance. However, such methods are often associated with high costs and are not always applicable to laboratories based in the least-developed countries. Here, we present and validate a low-cost screening protocol that can be used to differentiate between azole-susceptible “wild-type” and azole-resistant A. fumigatus isolates. © 2020 The Authors.

Basic Protocol 1: Preparation of Tebucheck multi-well plates

Basic Protocol 2: Inoculation of Tebucheck multi-well plates

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以低成本替布康唑为基础的耐唑烟曲霉筛选试验

烟曲霉对唑的耐药性在全球范围内的出现引起了健康和粮食安全方面的关注。快速诊断和环境监测方法是了解唑类耐药分布和流行的关键。然而，这种方法往往伴随着高昂的费用，而且并不总是适用于设在最不发达国家的实验室。在这里，我们提出并验证了一种低成本的筛选方案，该方案可用于区分对唑敏感的“野生型”和对唑耐药的烟曲霉分离株。©2020作者。基本程序1:制备Tebucheck多孔板基本程序2:接种Tebucheck多孔板

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Current Protocols in Microbiology Immunology and Microbiology-Parasitology

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期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.