A Transgenic System for Rapid Magnetic Enrichment of Rare Embryonic Cells.

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Zebrafish Pub Date : 2020-10-01 Epub Date: 2020-07-29 DOI:10.1089/zeb.2020.1904

Samuel J Wattrus, Leonard I Zon

引用次数: 3

Abstract

Collecting large numbers of rare cells for high-throughput molecular analysis remains a technical challenge, primarily due to limitations in existing technologies. In developmental biology this has impeded single-cell analysis of primordial organs, which derive from few cells. In this study, we share novel transgenic lines for rapid cell enrichment from zebrafish embryos using human surface antigens for immunological binding and magnetic sorting. As proof of principle, we tagged, enriched, and performed single-cell RNA sequencing on nascent hematopoietic stem/progenitor cells and endothelial cells from early embryos. Our method is a quick, efficient, and cost-effective approach to a previously intractable problem.

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一种稀有胚胎细胞快速磁富集的转基因系统。

由于现有技术的限制，收集大量稀有细胞进行高通量分子分析仍然是一项技术挑战。在发育生物学中，这阻碍了对源自少数细胞的原始器官的单细胞分析。在这项研究中，我们分享了一种新的转基因系，用于斑马鱼胚胎的快速细胞富集，利用人表面抗原进行免疫结合和磁分选。作为原理证明，我们对来自早期胚胎的新生造血干细胞/祖细胞和内皮细胞进行了标记、富集和单细胞RNA测序。我们的方法是一种快速、高效、经济的方法来解决以前难以解决的问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.