Comparison of Tissue Loading Before and After the Creation of a Continuous Density Gradient in Porcine Islet Purification.

Chika Miyagi-Shiohira, Yoshiki Nakashima, Nana Ebi, Eri Hamada, Yoshihito Tamaki, Kazuho Kuwae, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Takao Kinjo, Hirofumi Noguchi
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引用次数: 2

Abstract

The purification step is one of the most important and difficult procedures in islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from the porcine pancreas. In this study, we evaluated the impact of the timing of tissue loading on porcine islet purification using large plastic bottles. One method involved loading digested tissue after creating a continuous density gradient (tissue after gradient [TAG]). The other method involved loading digested tissue before creating a continuous density gradient (tissue before gradient [TBG]). There were no significant differences between TAG and TBG in terms of the islet yield, rates of viability and purity, score, and in the stimulation index after purification. Furthermore, there were no marked differences in the attainability or suitability of post-transplantation normoglycemia. Our study shows the equivalency of these two methods of islet purification.

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Abstract Image

Abstract Image

猪胰岛纯化中连续密度梯度产生前后组织负荷的比较。
纯化步骤是用于胰岛移植的胰岛分离中最重要和最困难的步骤之一。我们之前报道过,使用大塑料瓶的纯化方法有效地实现了猪胰腺胰岛的高产率。在本研究中,我们评估了组织加载时间对使用大塑料瓶纯化猪胰岛的影响。一种方法涉及在产生连续密度梯度(梯度后组织[TAG])后装载消化组织。另一种方法是在产生连续密度梯度(梯度前组织[TBG])之前加载消化组织。TAG和TBG在胰岛产量、存活率和纯度、评分以及纯化后的刺激指数方面没有显著差异。此外,移植后血糖正常的可及性或适用性没有显著差异。我们的研究表明这两种胰岛纯化方法是等效的。
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来源期刊
Cell medicine
Cell medicine MEDICINE, RESEARCH & EXPERIMENTAL-
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