Piperine from Black Pepper Decreased the Expression of Intercellular Adhesion Molecule-1 in Macrophages.

Q2 Medicine

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pub Date : 2021-01-01 DOI:10.2174/1871523019666200702093759

Nasser Gholijani, Esmaeil Hashemi, Zahra Amirghofran

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引用次数: 1

Abstract

Background: Macrophages are the main players involved in inflammation. Intercellular adhesion molecule-1 (ICAM-1) facilitates macrophage polarization prior to extravasation into inflamed tissue. Piperine, a natural product derived from black pepper, possesses useful biological and pharmacological activities. In the current study, the possible anti-inflammatory effect of piperine on the expression of ICAM-1 on J774.1 murine macrophage cell line was investigated.

Methods: Lipopolysaccharide (LPS)-stimulated J774.1 cells were cultured in the presence of different concentrations of piperine to examine the changes in ICAM-1 expression by real-time PCR and flow cytometry.

Results: We found that piperine decreased ICAM-1 gene expression level from 2.4 ± 0.25 RFC (relative fold change) in LPS-only treated cells to 0.85 ± 0.525 RFC at 1µg/ml (p<0.05), 0.43 ± 0.27 RFC at 10µg/ml (p<0.01), and 0.26 ± 0.25 RFC at 20µg/ml (p<0.01). In flow cytometry, piperine at all concentrations significantly decreased ICAM-1 surface expressions (P<0.05). The geometric mean fluorescence intensity (g-MFI) in LPS-only treated cells (792 ± 57.3) decreased to 482±70 g-MFI at 20 μg/ml piperine.

Conclusion: According to the results of this study, by decreasing the expression of ICAM-1, piperine has been suggested to reduce inflammation and have the potential to provide therapeutic benefits for immune-mediated diseases.

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黑胡椒胡椒碱降低巨噬细胞细胞间粘附分子-1的表达。

背景:巨噬细胞是炎症的主要参与者。细胞间粘附分子-1 (ICAM-1)促进巨噬细胞在外渗到炎症组织之前的极化。胡椒碱是一种从黑胡椒中提取的天然产物，具有良好的生物学和药理活性。本研究探讨胡椒碱对J774.1小鼠巨噬细胞ICAM-1表达的可能的抗炎作用。方法:将脂多糖(LPS)刺激的J774.1细胞在不同浓度胡椒碱的作用下培养，采用实时荧光定量PCR和流式细胞术检测ICAM-1表达的变化。结果:我们发现胡椒碱降低ICAM-1基因表达水平，从仅lps处理细胞的2.4±0.25 RFC(相对fold change)降低到1µg/ml时的0.85±0.525 RFC (pp结论:根据本研究结果，胡椒碱通过降低ICAM-1的表达，被认为可以减轻炎症，并有可能为免疫介导性疾病提供治疗益处。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

3.30

自引率

0.00%

发文量

期刊介绍： Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new anti-inflammatory & anti-allergy agents. Publishing a series of timely in-depth reviews written by leaders in the field covering a range of current topics, Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in the field.