Radioprotective Effect of Pioglitazone Against Genotoxicity Induced by Ionizing Radiation in Healthy Human Lymphocytes.

Q2 Medicine

Cardiovascular and Hematological Agents in Medicinal Chemistry Pub Date : 2021-01-01 DOI:10.2174/1871525718666200525005231

Roya Kazemi, Seyed J Hosseinimehr

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引用次数: 0

Abstract

Objective: Pioglitazone (PG) is used to control high blood sugar in patients with type 2 diabetes mellitus. PG acts as a peroxisome proliferator-activated receptor γ agonist. In addition to the insulin-sensitizing effect, PG possesses anti-inflammatory effect. In this study, the protective effect of PG was evaluated against DNA damage induced by ionizing radiation in healthy human lymphocytes.

Methods: The microtubes containing human whole blood were treated with PG at various concentrations (1-50 μM) for three hours. Then, the blood samples were irradiated with X-ray. Lymphocytes were cultured for determining the frequency of micronuclei as a genotoxicity biomarker in binucleated lymphocytes.

Results: The mean percentage of micronuclei was significantly increased in human lymphocytes when exposed to IR, while it was decreased in lymphocytes pre-treated with PG. The maximum reduction in the frequency of micronuclei in irradiated lymphocytes was observed at 5 μM of PG treatment (48% decrease).

Conclusion: The anti-inflammatory property suggested the mechanism action of PG for protecting human lymphocytes against genotoxicity induced by ionizing radiation.

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吡格列酮对正常人淋巴细胞电离辐射遗传毒性的辐射防护作用。

目的:应用吡格列酮(PG)控制2型糖尿病患者的高血糖。PG作为过氧化物酶体增殖物激活受体γ激动剂。PG除具有胰岛素增敏作用外，还具有抗炎作用。本研究评价了PG对电离辐射诱导的健康人淋巴细胞DNA损伤的保护作用。方法:用不同浓度PG (1 ~ 50 μM)处理含人全血的微管3 h。然后，用x射线照射血液样本。培养淋巴细胞，以确定微核作为遗传毒性生物标志物在双核淋巴细胞中的频率。结果:人淋巴细胞微核的平均百分比在红外照射下显著升高，而PG预处理后的淋巴细胞微核的平均百分比降低，在PG处理5 μM时，微核的频率降低幅度最大(降低48%)。结论:PG具有抗炎作用，具有保护人淋巴细胞免受电离辐射遗传毒性的作用机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cardiovascular and Hematological Agents in Medicinal Chemistry Medicine-Cardiology and Cardiovascular Medicine

CiteScore

2.70

自引率

0.00%

发文量

期刊介绍： Cardiovascular & Hematological Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new Cardiovascular & Hematological Agents. Each issue contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in Cardiovascular & Hematological medicinal chemistry. Cardiovascular & Hematological Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in cardiovascular & hematological drug discovery.