Tao Ke, Abel Santamaría, Alexey A. Tinkov, Julia Bornhorst, Michael Aschner
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引用次数: 3
Abstract
Caenorhabditis elegans is a free-living animal that is used as a powerful experimental model in biological sciences. The natural habitat of the animal are areas rich in material from rotting plants or fruits being decomposed by a growing number of microorganisms. The ecology of the natural habitat of C. elegans is a complex interactive network involving many species, including numerous types of bacteria, viruses, fungi, slugs, snails, and isopods, among which bacteria play multifaceted roles in the natural history of C. elegans. Under laboratory conditions, C. elegans is routinely cultured in a petri dish filled with solidified agar and seeded with Escherichia coli strain OP50, the latter offering an alternative model to study the interaction between bacteria and host. Because of the clear advantages of generating specific bacterial foods for mechanistic studies in C. elegans, it is important to develop a robust protocol to generate high-quality bacterial foods commensurate with experimental requirements. Based on previous work by us and others, herein we present a protocol on how to generate these optimal bacterial food–based research tools. © 2020 by John Wiley & Sons, Inc.
Basic Protocol 1: Preparing concentrated E. coli OP50
Basic Protocol 2: Titrating bacteria concentration
Basic Protocol 3: Generating dead bacterial food by heating
Basic Protocol 4: Generating dead bacterial food by antibiotics
Basic Protocol 5: Feeding C. elegans with bacterial foods in liquid
秀丽隐杆线虫产生细菌食物的毒理学研究
秀丽隐杆线虫是一种自由生活的动物,在生物科学中被用作强有力的实验模型。动物的自然栖息地是富含腐烂植物或水果的物质的地区,这些物质被越来越多的微生物分解。秀丽隐杆线虫自然栖息地的生态是一个复杂的多物种互动网络,包括多种细菌、病毒、真菌、鼻涕虫、蜗牛和等足类动物,其中细菌在秀丽隐杆线虫的自然史中起着多方面的作用。在实验室条件下,秀丽隐杆线虫在充满凝固琼脂的培养皿中常规培养,并接种大肠杆菌菌株OP50,后者为研究细菌与宿主的相互作用提供了另一种模型。由于在秀丽隐杆线虫的机制研究中产生特定的细菌食物具有明显的优势,因此制定一个强大的方案来产生符合实验要求的高质量细菌食物是很重要的。基于我们和其他人之前的工作,我们在这里提出了一个关于如何产生这些最佳的细菌食物研究工具的方案。©2020 by John Wiley &基本方案1:制备浓缩大肠杆菌op50基本方案2:滴定细菌浓度基本方案3:通过加热产生死细菌食物基本方案4:通过抗生素产生死细菌食物基本方案5:用液体细菌食物喂养秀丽隐杆线虫
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