Antifungal Mechanism of Rhodotorula mucilaginosa and Aureobasidium sp. nov. Isolated from Cerbera manghas L. against the Growth of Destructive Molds in Post Harvested Apples.

Q1 Agricultural and Biological Sciences
Dalia Sukmawati, Andisa Shabrina, Reni Indrayanti, Tri Handayani Kurniati, Muktiningsih Nurjayadi, Iman Hidayat, Shabrina Nida Al Husna, Nuniek Ina Ratnaningtyas, Hesham El Enshasy, Daniel Joe Dailin, Abd El-Latif Hesham
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引用次数: 3

Abstract

Background: Apples often experience postharvest damage due to being attacked by mold organisms. Several groups of molds such as Aspergillus sp., Penicilium expansum, Botrytis cinerea, and Venturia sp. can cause a serious postharvest disease exhibited as watery regions where areas of blue-green tufts of spores develop. Current methods using fungicides to control pathogenic fungi can cause resistance if applied in the long term. An alternative procedure using yeast as a biological agent has been found.

Objective: The aim of this study is to screen potential yeast, which has the ability to inhibit the growth of Aspergillus brasielensis (isolate A1) and Aspergillus flavus section flavi (isolate A17) isolated from apple fruits.

Methods: Antagonism test using YMA dual culture medium using in vitro assays and ITS rDNA identification were performed.

Results: The result showed that 3 out of 19 yeast isolated from Cerbera manghas L, T1, T3 and T4, demonstrated the potential ability as a biocontrol agent. ITS rDNA identification demonstrated that T1 has a similarity to Rhodotorula mucilaginosa while T3 and T4 were identified as Aureobasidium sp. nov. The 3 isolates exhibited the ability to reduce the growth of A. brasiliensis sensu lato better than dithane 0.3% with a Disease Incidence (DI) of 100% and a Disease Severity (DS) value of 45%. Only isolate T1 and T3 were able to reduce decay symptoms in apples inoculated with A. flavus sensu lato (with DO and DS were 100% and 25%, respectively) compared to dithane pesticides 0.3%.

Conclusion: This study indicated that competition between nutrients occurs between pathogenic molds and under-yeast in vitro and in vivo conditions. However, further studies in the future might be able to elucidate the 'killer' activity and interaction with the pathogen cells and the bio-product production using Rhodotorula mucilaginosa and Aureoubasidium namibiae strains to control postharvest diseases.

芒果粘红霉菌和小黑穗病菌对苹果采后破坏性霉菌生长的抑菌机理研究。
背景:苹果在收获后经常受到霉菌的侵害。有几组霉菌,如曲霉、扩张青霉、灰霉菌和文氏菌等,可引起严重的采收后疾病,表现为在水样区域出现蓝绿色孢子丛。目前使用杀菌剂控制病原真菌的方法如果长期使用会引起耐药性。已经发现了一种使用酵母作为生物制剂的替代程序。目的:从苹果果实中筛选具有抑制巴西曲霉(A1)和黄曲霉(A17)生长能力的潜在酵母菌。方法:采用体外试验和ITS rDNA鉴定,采用YMA双培养基进行拮抗试验。结果:从芒草中分离得到的19株酵母中,T1、T3和T4 3株具有潜在的防虫活性。ITS rDNA鉴定结果表明,T1与粘红酵母(Rhodotorula mucilaginosa)具有相似性,T3和T4鉴定为金黄色葡萄球菌(Aureobasidium sp. 11)。3株分离物对巴西红酵母(a . brasiliensis sensu lato)生长的抑制作用优于乙烷0.3%,病发率(Disease Incidence, DI)为100%,病重(Disease Severity, DS)为45%。与乙烷杀虫剂0.3%相比,只有分离物T1和T3能减轻接种黄曲霉后苹果的腐烂症状(DO和DS分别为100%和25%)。结论:本研究表明,在体外和体内条件下,病原菌和酵母下菌之间存在营养物质的竞争。然而,未来的进一步研究可能能够阐明“杀伤”活性和与病原菌细胞的相互作用,以及利用粘红酵母和纳米金孢子菌菌株控制采后病害的生物制品生产。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Recent patents on food, nutrition & agriculture
Recent patents on food, nutrition & agriculture Agricultural and Biological Sciences-Agronomy and Crop Science
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