Molecular cloning and characterization of GuHMGR, an HMG-CoA reductase gene from liquorice (Glycyrrhiza uralensis).

中国农学前沿 Pub Date : 2011-01-01 Epub Date: 2011-09-09 DOI:10.1007/s11703-011-1121-3

Chunying Ma, Chunsheng Liu, Wenquan Wang

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Abstract

A full length cDNA encoding HMGR (designated as GuHMGR) was isolated from liquorice (Glycyrrhiza uralensis) based on degenerated PCR and genome walking. The full length cDNA of GuHMGR was 2330 bp with a 1518-bp open reading frame (ORF) encoding a 505-aa polypeptide. Bioinformatics analysis indicated that there were two trans-membrane domains in GuHMGR. A molecular model of tertiary structure showed that GuHMGR is a novel HMGR with a similar spatial structure to other plant HMGRs. The deduced polypeptide of GuHMGR has an isoelectric point (pI) of 6.41 and a calculated molecular weight of about 54.7 kDa. Sequence comparison and phylogenetic tree analysis showed that GuHMGR had the highest homology with HMGRs from Pisum sativum and Medicago truncatula, indicating that GuHMGR belongs to the plant HMGR group. Expression analysis showed the similar amount of transcript level of GuHMGR in roots and leaves, suggesting that this gene was expressed constitutively in plants. Therefore, this novel HMGR gene would possibly provide a new strategy for studying the glycyrrhizin metabolism at the molecular level in the future.

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甘草（Glycyrrhiza uralensis）HMG-CoA 还原酶基因 GuHMGR 的分子克隆和特性鉴定。

根据退化的 PCR 和基因组步行，从甘草（Glycyrrhiza uralensis）中分离出编码 HMGR 的全长 cDNA（命名为 GuHMGR）。GuHMGR 的全长 cDNA 为 2330 bp，开放阅读框（ORF）为 1518 bp，编码 505-aa 的多肽。生物信息学分析表明，GuHMGR有两个跨膜结构域。三级结构分子模型显示，GuHMGR是一种新型的HMGR，其空间结构与其他植物HMGR相似。推导出的 GuHMGR 多肽等电点（pI）为 6.41，计算分子量约为 54.7 kDa。序列比较和系统发生树分析表明，GuHMGR与Pisum sativum和Medicago truncatula中的HMGR具有最高的同源性，表明GuHMGR属于植物HMGR类。表达分析表明，GuHMGR在根部和叶片中的转录水平相似，表明该基因在植物中是组成型表达的。因此，这个新的 HMGR 基因可能会为今后在分子水平上研究甘草酸苷代谢提供一种新的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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中国农学前沿

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