Current Understanding of the Mechanism of Water Oxidation in Photosystem II and Its Relation to XFEL Data.

IF 12.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Annual review of biochemistry Pub Date : 2020-06-20 Epub Date: 2020-03-24 DOI:10.1146/annurev-biochem-011520-104801
Nicholas Cox, Dimitrios A Pantazis, Wolfgang Lubitz
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引用次数: 91

Abstract

The investigation of water oxidation in photosynthesis has remained a central topic in biochemical research for the last few decades due to the importance of this catalytic process for technological applications. Significant progress has been made following the 2011 report of a high-resolution X-ray crystallographic structure resolving the site of catalysis, a protein-bound Mn4CaOx complex, which passes through ≥5 intermediate states in the water-splitting cycle. Spectroscopic techniques complemented by quantum chemical calculations aided in understanding the electronic structure of the cofactor in all (detectable) states of the enzymatic process. Together with isotope labeling, these techniques also revealed the binding of the two substrate water molecules to the cluster. These results are described in the context of recent progress using X-ray crystallography with free-electron lasers on these intermediates. The data are instrumental for developing a model for the biological water oxidation cycle.

光系统II中水氧化机理的最新认识及其与XFEL数据的关系。
在过去的几十年里,由于光合作用中的水氧化催化过程对技术应用的重要性,研究一直是生物化学研究的中心课题。2011年,一项高分辨率的x射线晶体结构报道了催化位点,一种蛋白质结合的Mn4CaOx配合物在水分解循环中通过了≥5个中间态,这一报道取得了重大进展。光谱技术与量子化学计算相辅相成,有助于理解酶促过程中所有(可检测的)状态下辅因子的电子结构。与同位素标记一起,这些技术还揭示了两个底物水分子与簇的结合。这些结果是在使用自由电子激光对这些中间体进行x射线晶体学的最新进展的背景下描述的。这些数据有助于开发生物水氧化循环模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Annual review of biochemistry
Annual review of biochemistry 生物-生化与分子生物学
CiteScore
33.90
自引率
0.00%
发文量
31
期刊介绍: The Annual Review of Biochemistry, in publication since 1932, sets the standard for review articles in biological chemistry and molecular biology. Since its inception, these volumes have served as an indispensable resource for both the practicing biochemist and students of biochemistry.
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