Combined effects of probiotic and chondroprotector during osteoarthritis in rats.

IF 4.3 4区医学 0 MEDICINE, GENERAL & INTERNAL

Panminerva medica Pub Date : 2020-06-01 Epub Date: 2020-03-19 DOI:10.23736/S0031-0808.20.03841-0

Oleksandr Korotkyi, Kateryna Dvorshchenko, Tetyana Falalyeyeva, Oksana Sulaieva, Nazarii Kobyliak, Ludovico Abenavoli, Sharmila Fagoonee, Rinaldo Pellicano, Liudmila Ostapchenko

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引用次数: 14

Abstract

Background: Osteoarthritis (OA) is a joint affection, defined by articular cartilage demolition, risks of which rise with age. The aim of this study was to compare the efficacy of chondroitin sulfate (CS) course and multistrain live probiotic (LP) administered alone or in combination on the expression of TLR-2, TLR-4, TNF-α and NF-κB in articular cartilage, subchondral bone and synovial membrane during OA in rats.

Methods: OA was induced in male rats by injecting monoiodoacetate (MIA) in right hind knee. Therapeutic groups received 3 mg/kg of chondroprotector (ChP) CS for 28 days and/or 140 mg/kg of LP diet for 14 days. The expression of TLR-2, TLR-4, TNF-α and NF-κB in articular cartilage, subchondral bone and synovial membrane were determined with immunohistochemical staining kits (Thermo Fisher Scientific).

Results: It was established that MIA injection is associated with long-term structural changes in joint tissues that corresponded to OA-like features and associated with activation of pathogen-recognizing molecules and proinflammatory signaling pathways expression. Separate therapy with ChP and probiotics slightly decreased OA score limiting cell death and subchondral bone resorption. However, these changes were not associated with a significant decrease in TLR-2, TLR-4, NF-kB and TNF-α expression. On the other hand, the combination of ChP and LP treatment significantly decreased OA score. This correlated with a decrease in TLR-2, TLR-4, NF-kB and TNF-α expression in chondrocytes and synovial cells.

Conclusions: The outcomes of our research prove that ChPs amplify the positive action of LPs in OA attenuation.

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益生菌和软骨保护剂对大鼠骨关节炎的联合作用。

背景:骨关节炎(OA)是一种关节病变，其定义为关节软骨破坏，其风险随着年龄的增长而增加。本研究旨在比较硫酸软骨素(CS)疗程和多菌株活益生菌(LP)单独或联合给药对骨性关节炎大鼠关节软骨、软骨下骨和滑膜中TLR-2、TLR-4、TNF-α和NF-κB表达的影响。方法:雄性大鼠右后膝注射单碘乙酸酯(MIA)诱导OA。治疗组给予3 mg/kg软骨保护剂(ChP) CS 28 d, 140 mg/kg LP饲粮14 d。采用免疫组化染色试剂盒(Thermo Fisher Scientific)检测关节软骨、软骨下骨和滑膜组织中TLR-2、TLR-4、TNF-α和NF-κB的表达。结果:证实MIA注射与关节组织的长期结构变化相关，这种结构变化符合oa样特征，并与病原体识别分子的激活和促炎信号通路的表达相关。单独使用ChP和益生菌治疗可轻微降低OA评分，限制细胞死亡和软骨下骨吸收。然而，这些变化与TLR-2、TLR-4、NF-kB和TNF-α表达的显著降低无关。另一方面，ChP与LP联合治疗可显著降低OA评分。这与软骨细胞和滑膜细胞中TLR-2、TLR-4、NF-kB和TNF-α表达的降低有关。结论:我们的研究结果证明，ChPs增强了LPs在OA衰减中的积极作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Panminerva medica 医学-医学：内科

CiteScore

5.00

自引率

2.30%

发文量

199

审稿时长

>12 weeks

期刊介绍： Panminerva Medica publishes scientific papers on internal medicine. Manuscripts may be submitted in the form of editorials, original articles, review articles, case reports, special articles, letters to the Editor and guidelines. The journal aims to provide its readers with papers of the highest quality and impact through a process of careful peer review and editorial work. Duties and responsibilities of all the subjects involved in the editorial process are summarized at Publication ethics. Manuscripts are expected to comply with the instructions to authors which conform to the Uniform Requirements for Manuscripts Submitted to Biomedical Editors by the International Committee of Medical Journal Editors (ICMJE).