Isolation and genomic analysis of 11-aminoundecanoic acid-degrading bacterium Pseudomonas sp. JG-B from nylon 11 enrichment culture.

Journal of Genomics Pub Date : 2020-01-25 eCollection Date: 2020-01-01 DOI:10.7150/jgen.42095
Jocelyn Gatz-Schrupp, Peter Deckard, Benjamin Hufford, Steven Ly, Peter Tupa, Hisako Masuda
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引用次数: 3

Abstract

Nylon 11 is a polymer synthesized from 11-aminoundecanoic acid, and widely used in commercial manufacturing. In this study, we describe the isolation of the first organism capable of metabolizing 11-aminoundecanoic acid from nylon 11 enrichment culture. The strain shows rapid growth on 11-aminoundecanoic acid as a sole source of carbon, nitrogen, and energy. Furthermore, the genome sequence of strain JG-B was deciphered and shown to belong to genus Pseudomonas. Many genes encoding putative extracellular hydrolases, as well as homologues of nylon 6 hydrolases (NylB and NylA) were identified, suggesting the metabolic versatility and possibility that this organism could also depolymerase nylon 11 polymers.

Abstract Image

Abstract Image

Abstract Image

尼龙11富集培养中11-氨基癸酸降解细菌Pseudomonas sp. JG-B的分离及基因组分析。
尼龙11是一种由11-氨基癸酸合成的聚合物,广泛用于商业生产。在这项研究中,我们描述了从尼龙11富集培养中分离出的第一个能够代谢11-氨基癸酸的生物。该菌株以11-氨基癸酸为唯一的碳、氮和能量来源,生长迅速。此外,菌株JG-B的基因组序列被破译,并显示属于假单胞菌属。许多基因编码假定的细胞外水解酶,以及尼龙6水解酶的同源物(NylB和NylA)被鉴定出来,表明这种生物的代谢多样性和可能也可以解聚尼龙11聚合物。
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来源期刊
自引率
0.00%
发文量
11
审稿时长
12 weeks
期刊介绍: Journal of Genomics publishes papers of high quality in all areas of gene, genetics, genomics, proteomics, metabolomics, DNA/RNA, computational biology, bioinformatics, and other relevant areas of research and application. Articles published by the journal are rigorously peer-reviewed. Types of articles include: Research paper, Short research communication, Review or mini-reviews, Commentary, Database, Software.
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