Purification of T Cell Populations

Abstract

This article describes a procedure for isolating T cell subpopulations using various methods including indirect panning and immunopanning by microarray. In these methods, cells are selected by their capacity to bind to antibody-coated plates (or slides) on the basis of particular cell-surface markers. Such methods can be superior to the antibody/complement lysis method (Alternate Protocol), as they can select additional cell population for analysis. © 2020 by John Wiley & Sons, Inc.

Basic Protocol 1: Isolation of T cell populations by indirect panning

Basic Protocol 2: Immunopanning with microarray

Alternate Protocol: Isolation of T cell populations by antibody/complement–mediated cytotoxicity