Risk Factors and Changes of Peripheral NK and T Cells in Pulmonary Interstitial Fibrosis of Patients with Rheumatoid Arthritis.

IF 2.1 4区医学 Q3 RESPIRATORY SYSTEM

Canadian respiratory journal Pub Date : 2019-12-01 eCollection Date: 2019-01-01 DOI:10.1155/2019/7262065

Na-Lin Lai, Wen Jia, Xia Wang, Jing Luo, Guang-Ying Liu, Chong Gao, Xiao-Feng Li, Jian-Fang Xie

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引用次数: 10

Abstract

Objective: The absolute and relative changes of peripheral NK and T subsets are unclear in rheumatoid arthritis (RA) associated with pulmonary interstitial fibrosis (RA-ILD). To investigate the clinical risk factors, especially the changes of lymphocyte subsets, in RA-ILD in order to make early diagnosis and achieve prevention of the pulmonary interstitial lesions.

Methods: A total of 100 RA and 100 RA-ILD patients were enrolled. Rheumatoid factor, anti-cyclic citrulline peptide antibody, erythrocyte sedimentation rate, immunoglobulin, and C-reactive protein were examined. The percentage and absolute number of NK, T, B, Treg, Th1, Th2, and Th17 cells in peripheral blood were determined by flow cytometry.

Results: RA-ILD is more common in older and male RA patients and/or those with higher autoantibody titers. Flow cytometry showed that the absolute and relative numbers of CD56+ NK cells were significantly higher in RA-ILD (280.40 ± 180.51 cells/μl vs. 207.66 ± 148.57 cells/μl; 16.62 ± 8.56% vs. 12.11 ± 6.47%), whereas the proportion of T cells and CD4+ T cells was lower in peripheral blood of RA-ILD patients (69.82 ± 9.30%; 39.44 ± 9.87 cells/μl) than that in RA patients (74.45 ± 8.72%; 43.29 ± 9.10 cells/μl).

Conclusions: The occurrence of RA-ILD is closely related to the older male patients with high titer of various self-antibodies. Imbalance of CD3-CD56+ NK cells and T cells with other subsets were found in RA-ILD patients, which, together with older age, male, and high levels of autoantibodies should be considered as risk factors of pulmonary interstitial lesions.

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类风湿关节炎患者肺间质纤维化的危险因素及外周血NK、T细胞的变化。

目的:类风湿关节炎(RA)合并肺间质纤维化(RA- ild)患者外周血NK和T亚群的绝对和相对变化尚不清楚。探讨RA-ILD的临床危险因素，特别是淋巴细胞亚群的变化，以期早期诊断和预防肺间质病变。方法:共纳入100例RA和100例RA- ild患者。检测类风湿因子、抗环瓜氨酸肽抗体、红细胞沉降率、免疫球蛋白、c反应蛋白。流式细胞术检测外周血NK、T、B、Treg、Th1、Th2、Th17细胞的百分比和绝对数量。结果:RA- ild更常见于老年和男性RA患者和/或自身抗体滴度较高的患者。流式细胞术显示，RA-ILD中CD56+ NK细胞的绝对数量和相对数量明显高于对照组(280.40±180.51个细胞/μl vs. 207.66±148.57个细胞/μl);(16.62±8.56% vs. 12.11±6.47%)，而RA-ILD患者外周血T细胞和CD4+ T细胞比例较低(69.82±9.30%;(39.44±9.87个细胞/μl)比RA组(74.45±8.72%;43.29±9.10 cells/μl)。结论:RA-ILD的发生与老年男性患者各种自身抗体高滴度密切相关。在RA-ILD患者中发现CD3-CD56+ NK细胞和T细胞与其他亚群的失衡，这与年龄较大、男性、自身抗体水平高应被认为是肺间质病变的危险因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Canadian respiratory journal 医学-呼吸系统

CiteScore

4.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Canadian Respiratory Journal is a peer-reviewed, Open Access journal that aims to provide a multidisciplinary forum for research in all areas of respiratory medicine. The journal publishes original research articles, review articles, and clinical studies related to asthma, allergy, COPD, non-invasive ventilation, therapeutic intervention, lung cancer, airway and lung infections, as well as any other respiratory diseases.