Magnetic Resonance Relaxometry for Determination of Protein Concentration and Aggregation

Q1 Biochemistry, Genetics and Molecular Biology

Current Protocols in Protein Science Pub Date : 2019-12-23 DOI:10.1002/cpps.102

Marc B. Taraban, Katharine T. Briggs, Yihua Bruce Yu

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引用次数: 6

Abstract

The water-proton signal, overwhelmingly considered a nuisance in nuclear magnetic resonance spectroscopy, is advantageously used as a tool to assess protein concentration and to detect protein aggregates in aqueous solutions. The protocols in this article describe use of the water-proton transverse relaxation rate to determine concentration and aggregate content in protein solutions. Detailed recommendations and description of the parameter settings and data processing ensure successful implementation of this technique, even by a user with limited experience in magnetic resonance relaxometry. All measurements are done noninvasively, in a sealed container, without sampling or otherwise aliquoting the solution. The magnetic resonance relaxometry approach offered in this article could be advantageous for analysis of biologics formulations or when use of conventional analytical techniques is not possible. © 2019 by John Wiley & Sons, Inc.

Basic Protocol 1: Nuclear magnetic resonance (NMR) relaxometry to measure protein concentration

Basic Protocol 2: NMR relaxometry to measure protein aggregation

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磁共振松弛法测定蛋白质浓度和聚集

水质子信号，在核磁共振波谱学中被认为是一种讨厌的东西，是一种有利的工具，用于评估蛋白质浓度和检测水溶液中的蛋白质聚集体。在这篇文章的协议描述使用水质子横向弛豫率来确定浓度和聚集含量在蛋白质溶液。参数设置和数据处理的详细建议和描述确保该技术的成功实施，即使是在磁共振松弛测量经验有限的用户。所有测量均在密封容器中无创完成，无需采样或以其他方式引用溶液。本文提供的磁共振弛豫测量方法可用于生物制剂的分析或当使用传统的分析技术是不可能的。©2019 by John Wiley &基本方案1:核磁共振弛豫仪测量蛋白质浓度基本方案2:核磁共振弛豫仪测量蛋白质聚集

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Current Protocols in Protein Science Biochemistry, Genetics and Molecular Biology-Biochemistry

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期刊介绍： With the mapping of the human genome, more and more researchers are exploring protein structures and functions in living organisms. Current Protocols in Protein Science provides protein scientists, biochemists, molecular biologists, geneticists, and others with the first comprehensive suite of protocols for this growing field.