Carbonyl Sulfide as a Prebiotic Activation Agent for Stereo- and Sequence-Selective, Amyloid-Templated Peptide Elongation.

IF 1.9 4区物理与天体物理 Q2 BIOLOGY

Origins of Life and Evolution of Biospheres Pub Date : 2019-12-01 Epub Date: 2019-12-16 DOI:10.1007/s11084-019-09586-5

Radoslaw Bomba, Saroj K Rout, Matthias Bütikofer, Witek Kwiatkowski, Roland Riek, Jason Greenwald

引用次数: 2

Abstract

Prebiotic chemical replication is a commonly assumed precursor to and prerequisite for life and as such is the one of the goals of our research. We have previously reported on the role that short peptide amyloids could have played in a template-based chemical elongation. Here we take a step closer to the goal by reproducing amyloid-templated peptide elongation with carbonyl sulfide (COS) in place of the less-prebiotically relevant carbonyldiimidazole (CDI) used in the earlier study. Our investigation shows that the sequence-selectivity and stereoselectivity of the amyloid-templated reaction is similar for both activation chemistries. Notably, the amyloid protects the peptides from some of the side-reactions that take place with the COS-activation.

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羰基硫化物作为立体和序列选择性淀粉样蛋白诱导肽延伸的前生物活化剂。

前生物化学复原通常被认为是生命的前身和先决条件，因此也是我们的研究目标之一。我们以前曾报道过短肽淀粉样蛋白在基于模板的化学延伸中可能发挥的作用。在这里，我们用硫化羰基（COS）取代了早先研究中使用的生物前相关性较低的羰基二咪唑（CDI），重现了淀粉样蛋白引发的肽延伸，从而向目标又迈进了一步。我们的研究表明，淀粉样蛋白诱导反应的序列选择性和立体选择性在两种活化化学物质中都是相似的。值得注意的是，淀粉样蛋白能保护肽免受 COS 活化过程中发生的一些副反应的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Origins of Life and Evolution of Biospheres 生物-生物学

CiteScore

3.20

自引率

15.00%

发文量

审稿时长

>12 weeks

期刊介绍： The subject of the origin and early evolution of life is an inseparable part of the general discipline of Astrobiology. The journal Origins of Life and Evolution of Biospheres places special importance on the interconnection as well as the interdisciplinary nature of these fields, as is reflected in its subject coverage. While any scientific study which contributes to our understanding of the origins, evolution and distribution of life in the Universe is suitable for inclusion in the journal, some examples of important areas of interest are: prebiotic chemistry and the nature of Earth''s early environment, self-replicating and self-organizing systems, the theory of the RNA world and of other possible precursor systems, and the problem of the origin of the genetic code. Early evolution of life - as revealed by such techniques as the elucidation of biochemical pathways, molecular phylogeny, the study of Precambrian sediments and fossils and of major innovations in microbial evolution - forms a second focus. As a larger and more general context for these areas, Astrobiology refers to the origin and evolution of life in a cosmic setting, and includes interstellar chemistry, planetary atmospheres and habitable zones, the organic chemistry of comets, meteorites, asteroids and other small bodies, biological adaptation to extreme environments, life detection and related areas. Experimental papers, theoretical articles and authorative literature reviews are all appropriate forms for submission to the journal. In the coming years, Astrobiology will play an even greater role in defining the journal''s coverage and keeping Origins of Life and Evolution of Biospheres well-placed in this growing interdisciplinary field.