Effective fraction of Bletilla striata reduces the inflammatory cytokine production induced by water and organic extracts of airborne fine particulate matter (PM_2.5) in vitro.

2区医学 Q1 Medicine

BMC Complementary and Alternative Medicine Pub Date : 2019-12-16 DOI:10.1186/s12906-019-2790-3

Yu-Yao Zu, Quan-Fang Liu, Shu-Xin Tian, Li-Xia Jin, Fu-Sheng Jiang, Mei-Ya Li, Bing-Qi Zhu, Zhi-Shan Ding

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Abstract

Background: Bletilla striata is a traditional Chinese medicine used to treat hemorrhage, scald, gastric ulcer, pulmonary diseases and inflammations. In this study, we investigated bioactivity of the effective fraction of B. striata (EFB) in reducing the inflammatory cytokine production induced by water or organic extracts of PM_2.5.

Methods: PM_2.5 extracts were collected and analyzed by chromatographic system and inductively coupled plasma mass spectrometer. Cell viability was measured using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay, and cell supernatant was analyzed by flow cytometry, ELISA, and qRT-PCR in cultured mouse macrophage cell line RAW264.7 treated with EFB and PM_2.5 extracts. Expressions of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathway were measured by Western blot.

Results: PM_2.5 composition is complex and the toxicity of PM_2.5 extracts were not noticeable. The treatment of EFB at a wide dose-range of 0-40 μg/mL did not cause significant change of RAW264.7 cell proliferation. EFB pretreatment decreased the inflammatory cytokines in the macrophage. Further analysis showed that EFB significantly attenuated PM_2.5-induced proinflammatory protein expression and downregulated the levels of phosphorylated NF-κBp65, inhibitor of kappa B (IκB)-α, c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and p38.

Conclusions: Our study demonstrated the potential effectiveness of B. striata extracts for treating PM_2.5-triggered pulmonary inflammation.

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条叶紫苏的有效成分可减少空气中细颗粒物（PM2.5）的水提取物和有机提取物在体外诱导的炎症细胞因子的产生。

背景：紫苏是一种传统中药，可用于治疗出血、烫伤、胃溃疡、肺部疾病和炎症。在本研究中，我们研究了条叶白芨有效成分（EFB）在减少水或有机提取物 PM2.5 诱导的炎症细胞因子产生方面的生物活性：方法：收集 PM2.5 提取物，用色谱系统和电感耦合等离子体质谱仪进行分析。用 MTS（3-(4,5-二甲基噻唑-2-基)-5-(3-羧基甲氧基苯基)-2-(4-磺酸苯基)-2H-四氮唑）法测定细胞活力，并用流式细胞仪、ELISA 和 qRT-PCR 分析经 EFB 和 PM2.5 提取物处理的小鼠巨噬细胞系 RAW264.7 的细胞上清液。核因子卡巴B（NF-κB）和丝裂原活化蛋白激酶（MAPK）信号通路的表达通过Western印迹法进行了测定：结果：PM2.5成分复杂，PM2.5提取物的毒性不明显。在 0-40 μg/mL 的宽剂量范围内处理 EFB 不会引起 RAW264.7 细胞增殖的显著变化。EFB 可降低巨噬细胞中的炎性细胞因子。进一步的分析表明，EFB能明显减轻PM2.5诱导的促炎蛋白表达，并下调磷酸化NF-κBp65、卡巴B抑制因子（IκB）-α、c-Jun N-末端激酶（JNK）、细胞外信号调节激酶（ERK）和p38的水平：我们的研究证明了B. striata提取物治疗PM2.5引发的肺部炎症的潜在功效。

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来源期刊

BMC Complementary and Alternative Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

7.00

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： BMC Complementary Medicine and Therapies is an open access journal publishing original peer-reviewed research articles on interventions and resources that complement or replace conventional therapies, with a specific emphasis on research that explores the biological mechanisms of action, as well as their efficacy, safety, costs, patterns of use and/or implementation.