The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines.

2区医学 Q1 Medicine

BMC Complementary and Alternative Medicine Pub Date : 2019-12-12 DOI:10.1186/s12906-019-2768-1

Carl Ammoury, Maria Younes, Marianne El Khoury, Mohammad H Hodroj, Tony Haykal, Peter Nasr, Marilyne Sily, Robin I Taleb, Rita Sarkis, Rana Khalife, Sandra Rizk

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引用次数: 0

Abstract

Background: The edible fruit Annona cherimola has previously shown many nutritional and medicinal properties. The current study evaluates the anti-cancer and anti-proliferative properties of Annona cherimola ethanolic leaf extract (AELE) on Acute Myeloid Leukemia (AML) cell lines cultured in vitro (Monomac-1 and KG-1).

Methods: The anti-proliferative effect of A. cherimola ethanolic leaf extract was evaluated via cell viability assay. Its pro-apoptotic effect was assessed through Cell Death ELISA and dual Annexin V/PI staining. To further investigate the molecular mechanism by which the extract promoted apoptosis and inhibited the proliferation of the AML cells used, apoptotic protein expression was determined through western blots. Extract composition was elucidated by Gas Chromatography-Mass Spectrometry (GC-MS).

Results: Our results showed that the treatment with A. cherimola ethanolic leaf extract exhibited an inhibitory effect on the proliferation of both cancer cell lines used in a dose- and time-dependent manner, with no toxic effects on normal mononuclear cells (MNCs) isolated from human bone marrow. This effect was mediated by DNA fragmentation and apoptosis, as revealed by Cell Death ELISA and dual Annexin V/PI staining. Western blot analysis revealed a Bax/Bcl2 dependent mechanism of apoptosis, as well as PARP cleavage, confirming the apoptotic results observed previously. These effects may be attributed to the presence of terpenes which constitute a large component of the leafy extract, as revealed via GC-MS.

Conclusion: All the data presented in our study show that the terpene-rich A. cherimola ethanolic leaf extract exhibits an anti-proliferative and pro-apoptotic effect on the AML cell lines used.

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富含萜烯的番荔枝叶提取物对白血病细胞系的促凋亡作用。

背景：番荔枝是一种可食用的水果，具有多种营养和药用特性。本研究评价番石榴乙醇叶提取物（AELE）对体外培养的急性髓细胞白血病（AML）细胞株（Monomac-1和KG-1）的抗癌和抗增殖作用。通过细胞死亡ELISA和双膜联蛋白V/PI染色评估其促凋亡作用。为了进一步研究提取物促进所用AML细胞凋亡和抑制其增殖的分子机制，通过蛋白质印迹测定了凋亡蛋白的表达。通过气相色谱-质谱（GC-MS）对提取物的组成进行了鉴定。结果：我们的结果表明，以剂量和时间依赖的方式使用番红花乙醇叶提取物处理对癌症细胞系的增殖具有抑制作用，对从人骨髓中分离的正常单核细胞（MNCs）没有毒性作用。细胞死亡ELISA和双膜联蛋白V/PI染色显示，这种作用是由DNA断裂和细胞凋亡介导的。Western印迹分析揭示了Bax/Bcl2依赖的细胞凋亡机制以及PARP切割，证实了先前观察到的细胞凋亡结果。这些作用可能归因于萜烯的存在，萜烯是叶提取物的主要成分，如GC-MS所揭示的。结论：我们研究中提供的所有数据表明，富含萜烯的番荔枝乙醇叶提取物对所使用的AML细胞系表现出抗增殖和促凋亡作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Complementary and Alternative Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

7.00

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： BMC Complementary Medicine and Therapies is an open access journal publishing original peer-reviewed research articles on interventions and resources that complement or replace conventional therapies, with a specific emphasis on research that explores the biological mechanisms of action, as well as their efficacy, safety, costs, patterns of use and/or implementation.