Comparison of Human Mesenchymal Stem Cells Derived from Various Compartments of Human Adipose Tissue and Tunica Adventitia Layer of the Arteries Subsequent to Organ Donation.

IF 0.3 Q4 TRANSPLANTATION

International Journal of Organ Transplantation Medicine Pub Date : 2019-01-01 Epub Date: 2019-05-01

M Kaviani, N Azarpira, M H Aghdaie, E Esfandiari, B Geramizadeh, S Nikeghbalian, M Dehghani

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引用次数: 0

Abstract

Background: Mesenchymal stem cells are one of the most interesting cell sources used in regenerative medicine.

Objective: In the present study, we isolated and characterized the mesenchymal stem cells from various compartments of human adipose tissue and tunica adventitia layer of the arteries.

Methods: Tissue explant culture was done from various compartments of the human adipose tissue and tunica adventitia layer of the arteries, including adipose tissue far from the vessels, perivascular tissues that are completely attached to the vessels, and tunica adventitia layer of the arteries. After the cell culture, characterization of the cells was determined at 3^rd-5^th passages. Flow cytometry was performed for antigen expression analysis of CD34, CD45, CD44, CD90, CD29, CD73, and CD105. For the evaluation of cell differentiation potential, adipogenic and osteogenic differentiation was conducted under appropriate protocols.

Results: The cells were positive for CD44, CD90, CD29, and CD73 and negative for CD34, CD45, and CD105. Adipogenic and osteogenic differentiation potentials were different among the cells from various compartments. The cells derived from perivascular tissue demonstrated better adipogenic and osteogenic differentiation.

Conclusion: It is essential to characterize the cells from different tissues and compartments for different purposes in regenerative medicine.

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人脂肪组织不同腔室及动脉外膜间充质干细胞捐献后的比较。

背景:间充质干细胞是再生医学中最有趣的细胞来源之一。目的:从人脂肪组织的不同腔室和动脉外膜中分离并鉴定间充质干细胞。方法:对人脂肪组织的各个腔室和动脉外膜层进行组织外植体培养，包括远离血管的脂肪组织、与血管完全附着的血管周围组织和动脉外膜层。细胞培养后，在第3 -5代时测定细胞的特性。流式细胞术检测CD34、CD45、CD44、CD90、CD29、CD73和CD105的抗原表达。为了评估细胞分化潜力，在适当的方案下进行了脂肪和成骨分化。结果:CD44、CD90、CD29、CD73表达阳性，CD34、CD45、CD105表达阴性。不同区室细胞的成脂和成骨分化潜能不同。来源于血管周围组织的细胞表现出更好的成脂性和成骨性分化。结论:在再生医学中，对不同组织和不同腔室的细胞进行表征具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Organ Transplantation Medicine TRANSPLANTATION-

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： The International Journal of Organ Transplantation Medicine (IJOTM) is a quarterly peer-reviewed English-language journal that publishes high-quality basic sciences and clinical research on transplantation. The scope of the journal includes organ and tissue donation, procurement and preservation; surgical techniques, innovations, and novelties in all aspects of transplantation; genomics and immunobiology; immunosuppressive drugs and pharmacology relevant to transplantation; graft survival and prevention of graft dysfunction and failure; clinical trials and population analyses in the field of transplantation; transplant complications; cell and tissue transplantation; infection; post-transplant malignancies; sociological and ethical issues and xenotransplantation.