Quantification of Cytoskeletal Dynamics in Time-Lapse Recordings

Q1 Agricultural and Biological Sciences

Current protocols in plant biology Pub Date : 2019-05-15 DOI:10.1002/cppb.20091

René Schneider, Arun Sampathkumar, Staffan Persson

引用次数: 7

Abstract

The cytoskeleton is key to many essential processes in a plant cell, e.g., growth, division, and defense. Contrary to what “skeleton” implies, the cytoskeleton is highly dynamic, and is able to re-organize itself continuously. The advent of live-cell microscopy and the development of genetically encoded fluorophores enabled detailed observation of the organization and dynamics of the cytoskeleton. Despite the biological importance of the cytoskeletal dynamics, quantitative analyses remain laborious endeavors that only a handful of research teams regularly conduct. With this protocol, we provide a standardized step-by-step guide to analyze the dynamics of microtubules. We provide example data and code for post-processing in Fiji that enables researchers to modify and adapt the routine to their needs. More such tools are needed to quantitatively assess the cytoskeleton and thus to better understand cell biology. © 2019 by John Wiley & Sons, Inc.

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定时记录中细胞骨架动力学的定量

细胞骨架是植物细胞许多重要过程的关键，如生长、分裂和防御。与“骨架”所暗示的相反，细胞骨架是高度动态的，并且能够不断地自我重组。活细胞显微镜的出现和基因编码荧光团的发展使得对细胞骨架的组织和动力学的详细观察成为可能。尽管细胞骨架动力学具有重要的生物学意义，但定量分析仍然是一项艰苦的工作，只有少数研究团队定期进行。有了这个协议，我们提供了一个标准化的分步指南来分析微管的动力学。我们为斐济的后处理提供了示例数据和代码，使研究人员能够根据他们的需要修改和调整程序。需要更多这样的工具来定量评估细胞骨架，从而更好地了解细胞生物学。©2019 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

自引率

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.