Lipolytic Enzymes with Hydrolytic and Esterification Activities Produced by Filamentous Fungi Isolated from Decomposition Leaves in an Aquatic Environment.

Q2 Biochemistry, Genetics and Molecular Biology
Enzyme Research Pub Date : 2019-06-02 eCollection Date: 2019-01-01 DOI:10.1155/2019/8182425
D B Mendes, F F Da Silva, P M Guarda, A F Almeida, D P de Oliveira, P B Morais, E A Guarda
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引用次数: 13

Abstract

Microbial lipases are prominent biocatalysts able to catalyze a wide variety of reactions in aqueous and nonaqueous media. In this work, filamentous fungi isolated from leaves decomposed in an aquatic environment were screened for lipase production with hydrolytic activity and esterification. Agar plates with Tween 20 and Rhodamine B were used for selection, while submerged cultures with olive oil were subsequently used to select 38 filamentous fungi. Trichoderma harzianum, Fusarium solani, Trichoderma harzianum F5, and Penicillium sp. F36 were grown in six different culture media. F. solani presented the highest lipase production (2.37 U/mL) with esterification activity of 0.07 U/mL using medium composed of (g.L-1) KH2PO4 1.00, MgSO4 H2O 1.123, and CuSO4 0.06. Supplementation of this culture medium with organic nitrogen sources increased lipase production by 461.3% using tryptone and by 419.4% using yeast extract. Among the vegetable oils from the Amazon region, degummed cotton oil induced lipase production up to 8.14 U/mL. The lipase produced by F. solani F61 has great potential to application in conventional processes and biodiesel production by transesterification of vegetable oils, as well as food industries in the production of fatty acid esters by hydrolysis and esterification.

Abstract Image

水生环境中从分解叶中分离的丝状真菌产生的具有水解和酯化活性的脂解酶。
微生物脂肪酶是一种突出的生物催化剂,能够在水性和非水性介质中催化各种反应。本工作从水生环境中分解的叶片中分离出丝状真菌,筛选出具有水解活性和酯化活性的脂肪酶。使用含有吐温20和罗丹明B的琼脂平板进行选择,而随后使用含有橄榄油的浸没培养物来选择38种丝状真菌。哈茨木霉、龙葵镰刀菌、哈茨木霉菌F5和青霉F36在六种不同的培养基中生长。使用由(g.L-1)KH2PO4 1.00、MgSO4 H2O 1.123和CuSO4 0.06组成的培养基,F.solani的脂肪酶产量最高(2.37U/mL),酯化活性为0.07U/mL。用有机氮源补充该培养基使用胰蛋白酶使脂肪酶产量增加461.3%,使用酵母提取物使脂肪酶产量提高419.4%。在亚马逊地区的植物油中,脱胶棉油诱导脂肪酶的产生高达8.14U/mL。由F.solani F61生产的脂肪酶在传统工艺和植物油酯交换生产生物柴油以及食品工业中水解和酯化生产脂肪酸酯方面具有巨大的应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Enzyme Research
Enzyme Research Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
4.60
自引率
0.00%
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