Determination of Dipicolinic Acid in "Natto" by High-Performance Liquid Chromatography Coupled With Postcolumn Photoirradiation With Zinc Acetate.

IF 4.1

International journal of tryptophan research : IJTR Pub Date : 2019-06-19 eCollection Date: 2019-01-01 DOI:10.1177/1178646919852120

Ken-Ichi Mawatari, Motomasa Atsumi, Fumiya Nakamura, Makoto Yasuda, Tomoko Fukuuchi, Noriko Yamaoka, Kiyoko Kaneko, Kazuya Nakagomi, Naoto Oku

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引用次数: 4

Abstract

A system was developed for determining dipicolinic acid in "natto" using liquid chromatography with fluorometric detection. The compound was separated by reversed-phase chromatography using a mobile phase of 0.1 mol/L disodium hydrogen phosphate, 0.05 mol/L citric acid buffer (adjusted to pH 3.0) containing 3.0 mmol/L zinc acetate and 35 mmol/L perchloric acid. The compound in the column effluent was irradiated with ultraviolet light to produce fluorescence. This fluorescence was monitored at an excitation at 336 nm and an emission at 448 nm. The calibration curve for dipicolinic acid was observed to be linear in a range of 0.2 to 112 ng. The dipicolinic acid content of natto was 7.24 ± 0.54 mg/100 g (wet weight, mean ± standard deviation [SD], n = 6).

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高效液相色谱-乙酸锌柱后光照射法测定纳豆中的二吡啶酸。

建立了液相色谱-荧光法测定纳豆中二吡啶酸的方法。以0.1 mol/L磷酸氢二钠、0.05 mol/L柠檬酸缓冲液(调节至pH 3.0)(含3.0 mmol/L乙酸锌和35 mmol/L高氯酸)为流动相，反相色谱法分离化合物。用紫外光照射柱出水中的化合物产生荧光。这种荧光在336 nm的激发和448 nm的发射处被监测。在0.2 ~ 112 ng范围内，二吡啶酸的校准曲线呈线性。纳豆中二吡啶酸含量为7.24±0.54 mg/100 g(湿重，平均值±标准差[SD]， n = 6)。

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International journal of tryptophan research : IJTR

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