Co-delivery of paclitaxel and curcumin to foliate positive cancer cells using Pluronic-coated iron oxide nanoparticles.

IF 4.4 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Progress in Biomaterials Pub Date : 2019-09-01 Epub Date: 2019-06-13 DOI:10.1007/s40204-019-0118-5
Chinmay G Hiremath, Geetha B Heggnnavar, Mahadevappa Y Kariduraganavar, Murigendra B Hiremath
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引用次数: 29

Abstract

Active targeting of folic acid and passive targeting of magnetic nanoparticles to bring about co-delivery of hydrophobic chemotherapeutic agents were the focus of this work. Co-precipitation in alkaline environment was employed for synthesizing Fe3O4 nanoparticles and stabilized by oleic acid. Aqueous dispersibility of oleic acid coated nanoparticles was brought about by folic acid modified Pluronic F127 and Pluronic F127 mixture. Folic acid is used as a targeting agent which was joined to Pluronic F127 via diethylene glycol bis(3-aminopropyl) ether spacer. The nanocomposite was used to delivery hydrophobic anticancer drugs, paclitaxel, and curcumin. Successful modification at each step was confirmed by FTIR and NMR. Quantitative analysis of attached folic acid indicated a total of 84.34% amount of conjugation. Nanoparticles characterization revealed the hydrodynamic size of and nanocomposite to be 94.2 nm nanometres. Furthermore, transmission electron micrograph reveals the size of the nanoparticle to be 12.5 nm hence also shows the superparamagnetic activity. Drug encapsulation efficiency of 34.7% and 59.5% was noted for paclitaxel and curcumin, respectively. Cytotoxic property of drug-loaded nanocomposites was increased in case of folic acid functionalized nanoparticles and further increased in the presence of an external magnetic field. Cellular uptake increased in the folic acid conjugated sample. Further many folds in the presence of an external magnetic field.

Abstract Image

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使用Pluronic-coated氧化铁纳米颗粒将紫杉醇和姜黄素共递送至叶片阳性癌症细胞。
叶酸的主动靶向和磁性纳米颗粒的被动靶向以实现疏水性化疗剂的共递送是本工作的重点。采用在碱性环境中共沉淀的方法合成了Fe3O4纳米粒子,并用油酸对其进行了稳定。油酸包被的纳米粒子的水分散性是通过叶酸改性的Pluronic F127和Pluronic F127的混合物实现的。使用叶酸作为靶向剂,其通过二甘醇双(3-氨基丙基)醚间隔物连接到Pluronic F127。该纳米复合材料用于递送疏水性抗癌药物、紫杉醇和姜黄素。FTIR和NMR证实了每个步骤的成功改性。所附叶酸的定量分析表明结合总量为84.34%。纳米颗粒表征显示纳米复合材料的流体动力学尺寸为94.2nm纳米。此外,透射电子显微照片显示纳米颗粒的尺寸为12.5nm,因此也显示出超顺磁活性。紫杉醇和姜黄素的药物包封率分别为34.7%和59.5%。在叶酸功能化的纳米颗粒的情况下,载药纳米复合材料的细胞毒性增加,并且在外部磁场的存在下进一步增加。叶酸结合样品中的细胞摄取增加。在存在外部磁场的情况下进一步折叠。
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来源期刊
Progress in Biomaterials
Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
9.60
自引率
4.10%
发文量
35
期刊介绍: Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.
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