{"title":"The rise of circulatory endothelin (ET)-1 and endothelin receptors (ET<sub>A</sub>, ET<sub>B</sub>) expression in kidney of obese wistar rat.","authors":"Irfan Idris, Andi Wardihan Sinrang, Aryadi Arsyad, Syafrudin Alwi, Muhammad Isman Sandira","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Endothelin (ET)-1, a circulatory protein, and its receptors (ET<sub>A</sub> and ET<sub>B</sub>) in various organs were reported to play a pivotal role in many diseases, including obesity. However, the changes of ET<sub>A</sub> and ET<sub>B</sub> expression in ventricle and kidney in obesity was less reported. The study is designed to observe the level of circulatory ET-1 and expression of ET<sub>A</sub>/ET<sub>B</sub> in ventricle and kidney of obese, as compared to non-obese, <i>Wistar</i> rats.</p><p><strong>Methods: </strong>Groups of obese 14 and 34 weeks <i>Wistar</i> rats were compared to non-obese controls at similar ages. The obesity status was achieved by feeding the with high calories protein diet CP 551 + milk powder, while the control group was fed with a standard calorie protein AD II diet. The concentration of circulatory ET-1, ET<sub>A</sub> and ET<sub>B</sub> of ventricle and kidney were measured by Enzyme Linked Immunosorbent Assay (ELISA) technique after the termination of both groups at 14<sup>th</sup> and 24<sup>th</sup> weeks.</p><p><strong>Results: </strong>The level of circulatory ET-1, expression of ET<sub>A</sub> and ET<sub>B</sub> in kidney, and LDL of obese rats were significantly higher than control rats (T-Test, P<0.05) in the elder groups, while no differences of the ET<sub>A</sub> and ET<sub>B</sub> were found in the ventricle. No differences of the levels of circulatory ET-1, ET<sub>A</sub> and ET<sub>B</sub> expression were found between obese and control groups of younger rats (P>0.05). HDL levels were under normal value for both groups.</p><p><strong>Conclusion: </strong>Obesity in elder obese rats leads to dysregulation of kidney vessels through activity of ET-1 and ET<sub>A</sub>/ET<sub>B</sub>.</p>","PeriodicalId":14352,"journal":{"name":"International journal of physiology, pathophysiology and pharmacology","volume":" ","pages":"31-35"},"PeriodicalIF":0.0000,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526386/pdf/ijppp0011-0031.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of physiology, pathophysiology and pharmacology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2019/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Background: Endothelin (ET)-1, a circulatory protein, and its receptors (ETA and ETB) in various organs were reported to play a pivotal role in many diseases, including obesity. However, the changes of ETA and ETB expression in ventricle and kidney in obesity was less reported. The study is designed to observe the level of circulatory ET-1 and expression of ETA/ETB in ventricle and kidney of obese, as compared to non-obese, Wistar rats.
Methods: Groups of obese 14 and 34 weeks Wistar rats were compared to non-obese controls at similar ages. The obesity status was achieved by feeding the with high calories protein diet CP 551 + milk powder, while the control group was fed with a standard calorie protein AD II diet. The concentration of circulatory ET-1, ETA and ETB of ventricle and kidney were measured by Enzyme Linked Immunosorbent Assay (ELISA) technique after the termination of both groups at 14th and 24th weeks.
Results: The level of circulatory ET-1, expression of ETA and ETB in kidney, and LDL of obese rats were significantly higher than control rats (T-Test, P<0.05) in the elder groups, while no differences of the ETA and ETB were found in the ventricle. No differences of the levels of circulatory ET-1, ETA and ETB expression were found between obese and control groups of younger rats (P>0.05). HDL levels were under normal value for both groups.
Conclusion: Obesity in elder obese rats leads to dysregulation of kidney vessels through activity of ET-1 and ETA/ETB.
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