Fabrication of biocompatible porous scaffolds based on hydroxyapatite/collagen/chitosan composite for restoration of defected maxillofacial mandible bone.

IF 4.4 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Progress in Biomaterials Pub Date : 2019-09-01 Epub Date: 2019-05-29 DOI:10.1007/s40204-019-0113-x
Md Shaifur Rahman, Md Masud Rana, Lucas-Sebastian Spitzhorn, Naznin Akhtar, Md Zahid Hasan, Naiyyum Choudhury, Tanja Fehm, Jan T Czernuszka, James Adjaye, Sikder M Asaduzzaman
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引用次数: 40

Abstract

Fabrication of scaffolds from biomaterials for restoration of defected mandible bone has attained increased attention due to limited accessibility of natural bone for grafting. Hydroxyapatite (Ha), collagen type 1 (Col1) and chitosan (Cs) are widely used biomaterials which could be fabricated as a scaffold to overcome the paucity of bone substitutes. Here, rabbit Col1, shrimp Cs and bovine Ha were extracted and characterized with respect to physicochemical properties. Following the biocompatibility, degradability and cytotoxicity tests for Ha, Col1 and Cs a hydroxyapatite/collagen/chitosan (Ha·Col1·Cs) scaffold was fabricated using thermally induced phase separation technique. This scaffold was cross-linked with (1) either glutaraldehyde (GTA), (2) de-hydrothermal treatment (DTH), (3) irradiation (IR) and (4) 2-hydroxyethyl methacrylate (HEMA), resulting in four independent types (Ha·Col1·Cs-GTA, Ha·Col1·Cs-IR, Ha·Col1·Cs-DTH and Ha·Col1·Cs-HEMA). The developed composite scaffolds were porous with 3D interconnected fiber microstructure. However, Ha·Col1·Cs-IR and Ha·Col1·Cs-GTA showed better hydrophilicity and biodegradability. All four scaffolds showed desirable blood biocompatibility without cytotoxicity for brine shrimp. In vitro studies in the presence of human amniotic fluid-derived mesenchymal stem cells revealed that Ha·Col1·Cs-IR and Ha·Col1·Cs-DHT scaffolds were non-cytotoxic and compatible for cell attachment, growth and mineralization. Further, grafting of Ha·Col1·Cs-IR and Ha·Col1·Cs-DHT was performed in a surgically created non-load-bearing rabbit maxillofacial mandible defect model. Histological and radiological observations indicated the restoration of defected bone. Ha·Col1·Cs-IR and Ha·Col1·Cs-DHT could be used as an alternative treatment in bone defects and may contribute to further development of scaffolds for bone tissue engineering.

羟基磷灰石/胶原/壳聚糖复合材料制备生物相容性多孔支架修复颌面部下颌骨缺损。
由于天然骨移植的可及性有限,用生物材料制作修复缺损下颌骨的支架越来越受到关注。羟基磷灰石(Ha)、1型胶原(Col1)和壳聚糖(Cs)是广泛使用的生物材料,可以作为支架来克服骨替代品的缺乏。在此,提取兔Col1、虾Cs和牛Ha,并对其理化性质进行表征。通过对Ha、Col1和Cs的生物相容性、降解性和细胞毒性测试,采用热诱导相分离技术制备了羟基磷灰石/胶原/壳聚糖(Ha·Col1·Cs)支架。该支架用(1)戊二醛(GTA)、(2)脱水热处理(DTH)、(3)辐照(IR)和(4)甲基丙烯酸2-羟基乙酯(HEMA)交联,得到四种独立的类型(Ha·Col1·Cs GTA、Ha·Col1·Cs IR、Ha·Col1·Cs DTH和Ha·Col1·Cs HEMA)。所开发的复合材料支架是多孔的,具有三维互连的纤维微观结构。而Ha·Col1·Cs IR和Ha·Col1·Cs GTA表现出较好的亲水性和生物降解性。所有四种支架都显示出良好的血液生物相容性,对卤虾没有细胞毒性。在人羊水来源的间充质干细胞存在下的体外研究表明,Ha·Col1·Cs IR和Ha·Col1·Cs DHT支架是非细胞毒性的,并且与细胞附着、生长和矿化相容。此外,在手术建立的非承载性兔颌面部下颌骨缺损模型中进行了Ha·Col1·Cs IR和Ha·Col1·Cs DHT的移植。组织学和放射学观察表明缺损骨已修复。Ha·Col1·Cs IR和Ha·Col1·Cs DHT可作为骨缺损的替代治疗方法,并有助于骨组织工程支架的进一步发展。
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来源期刊
Progress in Biomaterials
Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
9.60
自引率
4.10%
发文量
35
期刊介绍: Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.
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