Histopathological evaluation of mesenchymal stem cells in the healing of anastomosed carotid arteries.

IF 1.5 Q4 CELL BIOLOGY
American journal of stem cells Pub Date : 2019-04-15 eCollection Date: 2019-01-01
Adnan Altun, Cengiz Çokluk, Ercan Yarar, Enis Kuruoğlu, Keramettin Aydın, Eyüp Genç, Sezgin Gunes, Levent Yıldız, Abdullah Hilmi Marangoz
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Abstract

The objective of this study was to evaluate the influence of mesenchymal stem cells on the healing of experimental carotid artery anastomoses histopathologically. Twenty-four female Sprague-Dawley rats were used in this study. After random separation of the subjects into two groups, in both groups carotid arteries were transected and anastomosed in end-to-end fashion. Anastomoses were locally treated with 1 ml 0.09% NaCl, and 1 ml mesenchymal stem cell suspension (1×106 cells) in control and trial groups, respectively. Anastomoses were wrapped with an 8 mm sheet of surgicel and soaked with BioGlue in order to sequestrate the stem cells. After patencies were confirmed via Doppler USG, surgical site was closed with 2/0 silk sutures. Histopathological evaluation was carried out after 4 weeks. In respect to endothelial continuity, vessel patency (along with presence or absence of restenosis), integrities of internal and external elastic laminae, muscularis and adventitia; no statistically significant differences were present between the trial and control groups. In Trial and Control Groups, luminal thrombus was present in 8 (66.6%) and 3 (25%) of the 12 subjects, respectively. The difference was statistically significant (P < 0.05). Recanalization was present in 6 subjects in trial group; 1 subjects in Control Group, respectively. Our results suggest that local administration of mesenchyme stem cell does not have a positive influence on success of an anastomosis.

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间充质干细胞在颈动脉吻合愈合中的组织病理学评价。
本研究的目的是评价间充质干细胞对实验性颈动脉吻合口愈合的组织学影响。本研究选用24只雌性Sprague-Dawley大鼠。将受试者随机分为两组,两组均横切颈动脉,端到端进行吻合。对照组和试验组分别用1 ml 0.09% NaCl和1 ml间充质干细胞悬浮液(1×106细胞)局部处理吻合口。吻合口用8毫米厚的外科手术膜包裹,并用生物胶浸泡,以隔离干细胞。经多普勒USG确认通畅后,用2/0丝线缝合手术部位。4周后进行组织病理学评价。内皮连续性、血管通畅(有无再狭窄)、内外弹性板、肌层和外膜的完整性;试验组和对照组之间没有统计学上的显著差异。在试验组和对照组中,12例患者中分别有8例(66.6%)和3例(25%)出现腔内血栓。差异有统计学意义(P < 0.05)。试验组6例出现再通;对照组各1例。我们的结果表明,局部施用间充质干细胞对吻合的成功没有积极的影响。
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