Maricela Robles-Murguia, Liam C Hunt, David Finkelstein, Yiping Fan, Fabio Demontis
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引用次数: 16
Abstract
The GeneSwitch (GS) is a modified Gal4/UAS system, whereby transgene expression is induced in Drosophila by adding the drug RU486 to food. The GS system is routinely used in Drosophila aging and behavioral studies to avoid confounding effects related to genetic background mutations. Here, we report transcriptional and functional defects that are induced by RU486 in a stock- and tissue-dependent manner, such as defects in flight and mitochondrial gene expression. In addition to including proper controls, our findings suggest that context-specific side effects induced by RU486 should be considered in the experimental design and when interpreting the observed phenotypes.
GeneSwitch (GS)是一种改良的Gal4/UAS系统，通过将药物RU486添加到食物中来诱导果蝇的转基因表达。GS系统通常用于果蝇衰老和行为研究，以避免与遗传背景突变相关的混淆效应。在这里，我们报告了RU486以stock- and -组织依赖的方式诱导的转录和功能缺陷，例如飞行缺陷和线粒体基因表达缺陷。除了纳入适当的对照外，我们的研究结果表明，在实验设计和解释观察到的表型时，应考虑RU486诱导的情境特异性副作用。
期刊介绍：
npj Aging and Mechanisms of Disease is an online open access journal that provides a forum for the world’s most important research in the fields of aging and aging-related disease. The journal publishes papers from all relevant disciplines, encouraging those that shed light on the mechanisms behind aging and the associated diseases. The journal’s scope includes, but is not restricted to, the following areas (not listed in order of preference): • cellular and molecular mechanisms of aging and aging-related diseases • interventions to affect the process of aging and longevity • homeostatic regulation and aging • age-associated complications • translational research into prevention and treatment of aging-related diseases • mechanistic bases for epidemiological aspects of aging-related disease.
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