{"title":"Isolating Rat Intestinal Explants for In Vitro Cultures","authors":"Anjaney Kothari, Padmavathy Rajagopalan","doi":"10.1002/cptx.79","DOIUrl":null,"url":null,"abstract":"<p>The small intestine is an important organ primarily involved in digestion of food and absorption of nutrients. <i>In vitro</i> intestinal models are being developed to study this organ in health and disease. Intestinal explants can be used in such investigations since they contain all the major intestinal cell types. A detailed procedure to isolate intestinal explants from the rat jejunum is described. A protocol for culturing them <i>in vitro</i> for up to 24 hr is also provided. © 2019 by John Wiley & Sons, Inc.</p>","PeriodicalId":72743,"journal":{"name":"Current protocols in toxicology","volume":"80 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cptx.79","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols in toxicology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cptx.79","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
The small intestine is an important organ primarily involved in digestion of food and absorption of nutrients. In vitro intestinal models are being developed to study this organ in health and disease. Intestinal explants can be used in such investigations since they contain all the major intestinal cell types. A detailed procedure to isolate intestinal explants from the rat jejunum is described. A protocol for culturing them in vitro for up to 24 hr is also provided. © 2019 by John Wiley & Sons, Inc.
体外培养大鼠肠道外植体的分离
小肠是一个重要的器官，主要参与消化食物和吸收营养物质。正在开发体外肠道模型来研究这个器官在健康和疾病中的作用。肠道外植体可以用于此类调查，因为它们包含所有主要的肠道细胞类型。本文描述了从大鼠空肠中分离肠外植体的详细步骤。还提供了在体外培养它们长达24小时的方案。©2019 by John Wiley &儿子,Inc。
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