A novel model for ex situ reperfusion of the human liver following subnormothermic machine perfusion.

TECHNOLOGY Pub Date : 2017-12-01 Epub Date: 2017-11-10 DOI:10.1142/S2339547817500108
James H Avruch, Bote G Bruinsma, Pepijn D Weeder, Gautham V Sridharan, Robert J Porte, Heidi Yeh, James F Markmann, Korkut Uygun
{"title":"A novel model for ex situ reperfusion of the human liver following subnormothermic machine perfusion.","authors":"James H Avruch,&nbsp;Bote G Bruinsma,&nbsp;Pepijn D Weeder,&nbsp;Gautham V Sridharan,&nbsp;Robert J Porte,&nbsp;Heidi Yeh,&nbsp;James F Markmann,&nbsp;Korkut Uygun","doi":"10.1142/S2339547817500108","DOIUrl":null,"url":null,"abstract":"<p><p>Machine perfusion-based organ preservation techniques are prudently transitioning into clinical practice. Although experimental data is compelling, the outcomes in the highly variable clinical donation-transplantation setting are unpredictable. Here, we offer an intermediate tool for pre-clinical assessment of human donor livers. We present a model for ex situ reperfusion of discarded human livers and report on its application in three human livers that have undergone subnormothermic (21°C) machine perfusion as an experimental preservation method. During reperfusion, the livers macroscopically reperfused in the first 15 minutes, and remained visually well-perfused for 3 hours of ex situ reperfusion. Bile production and oxygen consumption were observed throughout ex situ reperfusion. ATP levels increased 4.25-fold during SNMP. Between the end of SNMP and the end of reperfusion ATP levels dropped 45%. ALT levels in blood increased rapidly in the first 30 minutes and ALT release continued to taper off towards the end of perfusion. Release of CRP, TNF-α, IL-1β, and IL-12, IFN-γ was sustained during reperfusion. These findings support the use of this model for the evaluation of novel human liver preservation techniques.</p>","PeriodicalId":22332,"journal":{"name":"TECHNOLOGY","volume":"5 4","pages":"196-200"},"PeriodicalIF":0.0000,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S2339547817500108","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"TECHNOLOGY","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1142/S2339547817500108","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/11/10 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

Abstract

Machine perfusion-based organ preservation techniques are prudently transitioning into clinical practice. Although experimental data is compelling, the outcomes in the highly variable clinical donation-transplantation setting are unpredictable. Here, we offer an intermediate tool for pre-clinical assessment of human donor livers. We present a model for ex situ reperfusion of discarded human livers and report on its application in three human livers that have undergone subnormothermic (21°C) machine perfusion as an experimental preservation method. During reperfusion, the livers macroscopically reperfused in the first 15 minutes, and remained visually well-perfused for 3 hours of ex situ reperfusion. Bile production and oxygen consumption were observed throughout ex situ reperfusion. ATP levels increased 4.25-fold during SNMP. Between the end of SNMP and the end of reperfusion ATP levels dropped 45%. ALT levels in blood increased rapidly in the first 30 minutes and ALT release continued to taper off towards the end of perfusion. Release of CRP, TNF-α, IL-1β, and IL-12, IFN-γ was sustained during reperfusion. These findings support the use of this model for the evaluation of novel human liver preservation techniques.

Abstract Image

Abstract Image

Abstract Image

一种新型的人肝脏离体再灌注模型。
基于机器灌注的器官保存技术正在谨慎地过渡到临床实践。虽然实验数据是令人信服的,结果在高度可变的临床捐献移植设置是不可预测的。在这里,我们提供了一种中间工具,用于临床前评估人类供体肝脏。我们提出了一种废弃人类肝脏的非原位再灌注模型,并报道了它在三个经过亚常温(21°C)机器灌注作为实验保存方法的人类肝脏中的应用。在再灌注过程中,肝脏在前15分钟内宏观上再灌注,并在3小时的非原位再灌注中保持视觉上的良好灌注。在离体再灌注过程中观察胆汁生成和耗氧量。ATP水平在SNMP期间增加了4.25倍。从SNMP结束到再灌注结束,ATP水平下降了45%。血液中ALT水平在前30分钟迅速升高,在灌注结束时ALT释放继续逐渐减少。再灌注过程中CRP、TNF-α、IL-1β、IL-12、IFN-γ持续释放。这些发现支持使用该模型评估新的人类肝脏保存技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
TECHNOLOGY
TECHNOLOGY ENGINEERING, MULTIDISCIPLINARY-
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信