E74 like ETS transcription factor 3 (ELF3) is a negative regulator of epithelial- mesenchymal transition in bladder carcinoma.

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Cancer biomarkers : section A of Disease markers Pub Date : 2019-01-01 DOI:10.3233/CBM-190013

Kirti Gondkar, Krishna Patel, Shobha Krishnappa, Akkamahadevi Patil, Bipin Nair, Gopinath Meenakshi Sundaram, Tan Tuan Zea, Prashant Kumar

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引用次数: 8

Abstract

Background: Transcription factors are commonly deregulated in various cancers. Here, we evaluated role of ELF3 in pathogenesis of bladder carcinoma (BCa).

Materials and methods: We confirmed ELF3 expression pattern in BCa cell lines using western blot; and in different grades of tumors using Immunohistochemistry. Cell invasion assay was employed to demonstrate potential role of ELF3 in EMT.

Results and conclusion: ELF3 showed selective expression in low-grade cell lines and tumor tissues. Overexpression of ELF3 in mesenchymal cell line UMUC3 resulted in reduced invasion and decreased expression of mesenchymal markers. We observed association of low ELF3 expression with increased risk and overall poor survival using publicly available data. ELF3-modulated reversal of EMT might be a useful strategy in the treatment of bladder cancer.

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E74样ETS转录因子3 (ELF3)是膀胱癌上皮-间质转化的负调控因子。

背景:转录因子在各种癌症中普遍失调。在此，我们评估ELF3在膀胱癌(BCa)发病机制中的作用。材料和方法:采用western blot法证实ELF3在BCa细胞株中的表达模式;在不同级别的肿瘤中使用免疫组织化学细胞侵袭实验证实ELF3在EMT中的潜在作用。结果与结论:ELF3在低分级细胞系和肿瘤组织中选择性表达。在间充质细胞系UMUC3中过表达ELF3导致侵袭减少，间充质标志物表达减少。我们使用公开数据观察到低ELF3表达与风险增加和总体生存不良之间的关联。elf3调控的EMT逆转可能是治疗膀胱癌的一种有效策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cancer biomarkers : section A of Disease markers

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