Assessing Blood Clotting and Coagulation Factors in Mice

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2019-03-15 DOI:10.1002/cpmo.61

Marisa A. Brake, Lacramioara Ivanciu, Susan A. Maroney, Nicolas D. Martinez, Alan E. Mast, Randal J. Westrick

引用次数: 19

Abstract

The mammalian blood coagulation system was designed to restrict blood loss due to injury as well as keep the blood fluid within the blood vessels of the organism. Blood coagulation activity in inbred mouse strains varies widely among strains, suggesting that many genomic variants affect hemostasis. Some of these molecules have been discovered and characterized; however, many are still unknown. Genetically modified mouse technologies are providing a plethora of new mouse models for investigating the regulation of blood coagulation. Here we provide a protocol for the tail bleeding time as a primary assessment of in vivo blood coagulation, as well as in vitro methods such as the prothrombin time, activated partial thromboplastin time, and thrombin generation assay. We also provide protocols for the assessment of the activities of specific known factors involved in blood coagulation. © 2019 by John Wiley & Sons, Inc.

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小鼠凝血和凝血因子的测定

哺乳动物血液凝固系统的设计是为了限制因受伤而导致的失血，并将血液液体保持在生物体的血管内。近交系小鼠的凝血活性在各系之间差异很大，表明许多基因组变异影响止血。其中一些分子已经被发现并被表征;然而，许多仍是未知的。转基因小鼠技术为研究凝血调节提供了大量新的小鼠模型。在这里，我们提供了一个方案，尾巴出血时间作为体内血液凝固的主要评估，以及在体外的方法，如凝血酶原时间，活化部分凝血活酶时间，凝血酶生成测定。我们还提供方案，以评估特定的已知因素参与血液凝固的活动。©2019 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.