Anti-inflammatory and Antioxidant Effects of Lotus corniculatus on Paracetamol-induced Hepatitis in Rats.

Q2 Medicine

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pub Date : 2020-01-01 DOI:10.2174/1871523018666190222120752

Nacera Baali, Abderahmane Mezrag, Mohamed Bouheroum, Fadila Benayache, Samir Benayache, Amedah Souad

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引用次数: 8

Abstract

Background: Herbal medicines have been used in the treatment of liver diseases for a long time. The current study was elaborated to evaluate in vitro and in vivo antioxidant and anti-inflammatory effects of Lotus corniculatus (L. corniculatus) butanolic extract.

Methods: The in vitro antioxidant and anti-inflammatory properties of L. corniculatus were investigated by employing DPPH radical scavenging, H2O2 scavenging and BSA denaturation assays. In vivo antioxidant and anti-inflammatory effects of L. corniculatus were evaluated against paracetamol (APAP)-induced hepatitis in rats. L.corniculatus at doses of 100 and 200 mg/kg was administered orally once daily for seven days. Serum transaminases (AST and ALT) and lactate dehydrogenase (LDH), total bilirubin levels, liver malondialdehyde (MDA), reduced glutathione (GSH), glutathione S- transferase (GST) and superoxide dismutase (SOD) levels and inflammatory markers, such as serum Creactive protein (CRP), circulating and liver myeloperoxidase (MPO) levels were investigated. Further histopathological analysis of the liver sections was performed to support the effectiveness of L. corniculatus.

Results: L. corniculatus exhibited strong antioxidant and anti-inflammatory effects in vitro. In the in vivo study, our findings demonstrate that L. corniculatus (100 and 200 mg/kg) administration led to an amelioration of APAP effects on liver histology, liver functions parameters (AST, ALT, LDH, and total bilirubin levels) and liver oxidative stress markers (MDA, GSH, GST and SOD levels). Furthermore, serum CRP, circulating MPO and liver MPO levels were declined by both doses of L. corniculatus extract. The best benefits were observed with 200 mg/kg of L. corniculatus extract.

Conclusion: Antioxidant and anti-inflammatory effects of L. corniculatus extract may be due to the presence of active components.

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白莲对扑热息痛所致大鼠肝炎的抗炎、抗氧化作用。

背景:草药用于肝脏疾病的治疗由来已久。本研究旨在评价金莲丁醇提取物的体内外抗氧化和抗炎作用。方法:采用清除DPPH自由基、清除H2O2、变性牛血清白蛋白的方法，研究白藜芦醇的体外抗氧化和抗炎作用。研究了白藜芦醇(L. corniculatus)对扑热息痛(APAP)所致大鼠肝炎的体内抗氧化和抗炎作用。给药剂量分别为100和200 mg/kg，每日口服1次，连用7天。检测血清转氨酶(AST、ALT)和乳酸脱氢酶(LDH)、总胆红素水平、肝脏丙二醛(MDA)、还原型谷胱甘肽(GSH)、谷胱甘肽S-转移酶(GST)和超氧化物歧化酶(SOD)水平以及血清活性蛋白(CRP)、循环和肝髓过氧化物酶(MPO)水平等炎症标志物。进一步的肝切片组织病理学分析，以支持牛角菌的有效性。结果:山茱萸具有较强的体外抗氧化和抗炎作用。在体内研究中，我们的研究结果表明，L. corniculatus(100和200 mg/kg)可改善APAP对肝脏组织学、肝功能参数(AST、ALT、LDH和总胆红素水平)和肝脏氧化应激标志物(MDA、GSH、GST和SOD水平)的影响。血清CRP、循环MPO和肝脏MPO水平均降低。以200 mg/kg的剂量效果最佳。结论:山茱萸提取物的抗氧化和抗炎作用可能与其中的活性成分有关。

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来源期刊

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

3.30

自引率

0.00%

发文量

期刊介绍： Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new anti-inflammatory & anti-allergy agents. Publishing a series of timely in-depth reviews written by leaders in the field covering a range of current topics, Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in the field.