Carnosol, a dietary diterpene from rosemary (Rosmarinus officinalis) activates Nrf2 leading to sestrin 2 induction in colon cells.

Integrative molecular medicine Pub Date : 2018-08-01 Epub Date: 2018-08-15 DOI:10.15761/IMM.1000335
Miao Yan, Bhaskar Vemu, Jacob Veenstra, Sakina M Petiwala, Jeremy J Johnson
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引用次数: 17

Abstract

Background: Rosemary is abundant with phytochemicals and has recently been approved as an antioxidant food preservative in the European Union. The safety of rosemary is well established, however, the benefits on gastrointestinal health are less known. Our overall hypothesis is that the phytochemicals in rosemary including carnosol have the potential to promote gastrointestinal health by activation of the antioxidant sestrin-2 when consumed in our diet.

Methods: Colon cells HCT116 and SW480 were treated with carnosol and evaluated by MTT, immunofluorescence, ELISA, and Western blot analysis to understand the modulation of the PERK/Nrf2/Sestrin-2 pathway.

Results: Carnosol was found to modulate PERK and increase the concentration of nuclear Nrf2. Furthermore, a downstream marker of Nrf2 expression, Sestrin-2 was shown to be upregulated.

Conclusion: Based on these observations carnosol modulates the PERK and Nrf2 pathways along with increased expression of sestrin-2, a known stress inducible antioxidant.

Abstract Image

Abstract Image

Abstract Image

从迷迭香(Rosmarinus officinalis)中提取的膳食二萜Carnosol可以激活Nrf2,从而诱导结肠细胞中的sestrin 2。
背景:迷迭香含有丰富的植物化学物质,最近在欧盟被批准为抗氧化食品防腐剂。迷迭香的安全性是公认的,然而,它对胃肠道健康的益处却鲜为人知。我们的总体假设是,迷迭香中的植物化学物质,包括鼠尾草醇,在我们的饮食中摄入时,有可能通过激活抗氧化剂sestrin-2来促进胃肠道健康。方法:用鼠油醇处理结肠癌细胞HCT116和SW480,通过MTT、免疫荧光、ELISA和Western blot分析,了解PERK/Nrf2/Sestrin-2通路的调节情况。结果:鼠尾油醇可调节PERK,增加核Nrf2的浓度。此外,Nrf2表达的下游标记物Sestrin-2被显示上调。结论:基于这些观察结果,鼠尾油醇可以调节PERK和Nrf2通路,同时增加应激诱导抗氧化剂sestrin-2的表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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