Association of A-197G polymorphism in interleukin-17 gene with chronic periodontitis: Evidence from six case-control studies with a computational biology approach

Q1 Medicine

Journal of Investigative and Clinical Dentistry Pub Date : 2019-06-23 DOI:10.1111/jicd.12424

Amir Farmohammadi, Atefeh Tavangar, Mohammad Ehteram, Mohammad Karimian

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引用次数: 4

Abstract

Aim

The aim of the present study was to evaluate the association of interleukin-17 (IL-17) A-197G gene polymorphism with chronic periodontitis (CP) in a case-control study, a meta-analysis, and an in silico approach.

Methods

In the case-control study, 122 cases with CP and 126 healthy controls were recruited; IL-17 A-197G genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism. In the meta-analysis, comprehensive literature retrieval was performed on valid databases to identify relevant studies. Bioinformatics tools were employed to investigate the effects of A-197G transition on the promoter region of IL-17.

Results

Our case-control study revealed a significant association between IL-17 A-197G transition and CP. The overall meta-analysis revealed significant associations between the IL-17 A-197G polymorphism and CP risk in homozygote co-dominant and recessive models. The stratified analysis also showed a statistically significant association between the mentioned transition and CP risk in the Caucasian population. The in silico analysis revealed that the A-197G polymorphism could make changes in protein-binding sites of the IL-17 promoter region.

Conclusions

Our study supports that IL-17 A-197G transition could be a genetic risk factor for CP. However, further studies with a larger sample size among different ethnicities are required to obtain a more accurate conclusion.

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白细胞介素-17基因a - 197g多态性与慢性牙周炎的关系:来自六个病例对照研究的证据，采用计算生物学方法

本研究的目的是通过病例对照研究、荟萃分析和计算机方法来评估白细胞介素-17 (IL-17) a- 197g基因多态性与慢性牙周炎(CP)的关系。方法采用病例-对照研究方法，选取CP患者122例，健康对照126例;采用聚合酶链反应-限制性片段长度多态性对il - 17a - 197g进行基因分型。在meta分析中，对有效的数据库进行全面的文献检索，找出相关的研究。利用生物信息学工具研究A-197G转化对IL-17启动子区的影响。结果我们的病例对照研究显示IL-17 a- 197g转化与CP之间存在显著相关性。总体荟萃分析显示，在纯合子共显性和隐性模型中，IL-17 a- 197g多态性与CP风险之间存在显著相关性。分层分析还显示，上述转变与高加索人群CP风险之间存在统计学上显著的关联。计算机分析显示，A-197G多态性可以改变IL-17启动子区蛋白结合位点。结论我们的研究支持IL-17 a - 197g转换可能是CP的遗传危险因素，但需要在不同种族中进行更大样本量的进一步研究才能获得更准确的结论。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Investigative and Clinical Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： Journal of Investigative and Clinical Dentistry (JICD) aims to publish quality, peer reviewed original research and topical reviews on all aspects of investigative and clinical dentistry and craniofacial research, including molecular studies related to oral health and disease. Although international in outlook, the Editor especially encourages papers from the Asia Pacific. The journal also aims to provide clinicians, scientists and students of dentistry with a knowledge transfer platform for rapid publication of reports through an international journal, which will be available free online until 2012. Its scope, therefore, is broad, inclusive and international, but with a particular focus on Asia Pacific. The Editor welcomes manuscripts in the following key thematic areas in oral and maxillofacial sciences: Community Dentistry and Oral Epidemiology, Conservative Dentistry, Dental Biomaterials, Dental Pedagogy, Endodontics and Traumatology, Implant Dentistry, Oral Biosciences, Oral and Maxillofacial Surgery, Oral Medicine, Oral Pathology and Oral Microbiology, Orthodontics, Oral Radiology, Oral Rehabilitation, Paedodontics, Periodontology and Periodontal Medicine.