Targeting RNA helicase DDX3 in stem cell maintenance and teratoma formation.

Q2 Biochemistry, Genetics and Molecular Biology
Candace L Kerr, Guus M Bol, Farhad Vesuna, Venu Raman
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引用次数: 10

Abstract

DDX3 is an RNA helicase that has antiapoptotic properties, and promotes proliferation and transformation. Besides the role of DDX3 in transformed cells, there is evidence to indicate that DDX3 expression is at its highest levels during early embryonic development and is also expressed in germ cells of adults. Even though there is a distinct pattern of DDX3 expression during embryonic development and in adults, very little is known regarding its role in embryonic stem cells and pluripotency. In this work, we examined the relationship between DDX3 and human embryonic stem cells and its differentiated lineages. DDX3 expression was analyzed by immunohistochemistry in human embryonic stem cells and embryonal carcinoma cells. From the data obtained, it was evident that DDX3 was overexpressed in undifferentiated stem cells compared to differentiated cells. Moreover, when DDX3 expression was abrogated in multiple stem cells, proliferation was decreased, but differentiation was facilitated. Importantly, this resulted in reduced potency to induce teratoma formation. Taken together, these findings indicate a distinct role for DDX3 in stem cell maintenance.

Abstract Image

Abstract Image

Abstract Image

靶向RNA解旋酶DDX3在干细胞维持和畸胎瘤形成中的作用。
DDX3是一种具有抗凋亡特性的RNA解旋酶,可促进细胞增殖和转化。除了DDX3在转化细胞中发挥作用外,有证据表明,DDX3在胚胎早期发育时表达水平最高,在成体生殖细胞中也有表达。尽管DDX3在胚胎发育和成人中有独特的表达模式,但对其在胚胎干细胞和多能性中的作用知之甚少。在这项工作中,我们研究了DDX3与人胚胎干细胞及其分化谱系的关系。免疫组化法分析DDX3在人胚胎干细胞和胚胎癌细胞中的表达。从获得的数据可以明显看出,与分化细胞相比,DDX3在未分化干细胞中过表达。此外,当DDX3在多干细胞中表达时,增殖减少,但分化促进。重要的是,这导致了诱导畸胎瘤形成的效力降低。综上所述,这些发现表明DDX3在干细胞维持中具有独特的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genes and Cancer
Genes and Cancer Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
3.90
自引率
0.00%
发文量
6
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