Anti-apoptotic traits in gingival tissue from patients with severe generalized chronic periodontitis

Q1 Medicine

Journal of Investigative and Clinical Dentistry Pub Date : 2019-06-13 DOI:10.1111/jicd.12422

Carlos Marcelo Figueredo, Juliana Cardoso Alves, Tatiane Flôr Coelho de Souza Breves Beiler, Ricardo Guimarães Fischer

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引用次数: 4

Abstract

Aim

In the present study, we aimed to evaluate the cytosolic and nuclear-mitochondrial expression of pro-apoptotic and anti-apoptotic markers in gingival tissue from patients with severe generalized chronic periodontitis (sGCP).

Methods

Twenty-four patients participated in the study: 15 (54.3 + 8.3 years) with sGCP and nine (38.2 + 5.4 years) with gingivitis alone. Gingival tissue was collected using a 1.5-mm diameter punch and homogenized using a cell disruptor. The supernatants were analyzed for the cytosolic and nuclear-mitochondrial fractions of caspase-3, Bax, Bak, Smac, lamin B, Bad, Bim, survivin, Bcl-xL, Mcl-1, and of the dimers Bcl-2/Bax, Bcl-xL/Bak and Mcl-1/Bak using a multiplex immunoassay.

Results

Significantly higher levels of cytosolic Bcl-xL/Bak, nuclear-mitochondrial Mcl-1/Bak, and cytosolic Bcl-xL were observed in gingival tissues from periodontitis patients compared to controls (P = 0.03, 0.03, and 0.05, respectively). The patients with gingivitis presented significantly increased levels of nuclear-mitochondrial Bad, cytosolic and nuclear-mitochondrial Bcl-2/Bax, and cytosolic Bim compared to the patients with periodontitis (P < 0.001, 0.03, 0.05, and 0.04, respectively).

Conclusion

Significantly higher levels of anti-apoptotic markers, such as Bcl-xL/Bak, Mcl-1/Bak, and Bcl-xL, and lower levels of pro-apoptotic markers Bad and Bim in inflamed tissues indicate an anti-apoptotic trait in patients with sGCP.

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重度全身性慢性牙周炎患者牙龈组织的抗凋亡特性

目的研究重度广泛性慢性牙周炎(sGCP)患者牙龈组织中促凋亡和抗凋亡标志物的胞浆和核线粒体表达。方法24例患者中，15例(54.3 + 8.3年)为sGCP, 9例(38.2 + 5.4年)为单纯牙龈炎。使用直径1.5 mm的穿孔器收集牙龈组织，并使用细胞干扰剂均匀化。利用多重免疫分析法分析上清液中caspase-3、Bax、Bak、Smac、lamin B、Bad、Bim、survivin、Bcl-xL、Mcl-1以及二聚体Bcl-2/Bax、Bcl-xL/Bak和Mcl-1/Bak的胞浆和核线粒体组分。结果牙周炎患者牙龈组织中胞浆Bcl-xL/Bak、核线粒体Mcl-1/Bak和胞浆Bcl-xL水平明显高于对照组(P值分别为0.03、0.03和0.05)。牙龈炎患者与牙周炎患者相比，核线粒体Bad、胞质和核线粒体Bcl-2/Bax、胞质Bim水平显著升高(P <0.001、0.03、0.05和0.04)。结论sGCP患者炎症组织中抗凋亡标志物Bcl-xL/Bak、Mcl-1/Bak、Bcl-xL水平升高，促凋亡标志物Bad、Bim水平降低，提示sGCP患者具有抗凋亡特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Investigative and Clinical Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： Journal of Investigative and Clinical Dentistry (JICD) aims to publish quality, peer reviewed original research and topical reviews on all aspects of investigative and clinical dentistry and craniofacial research, including molecular studies related to oral health and disease. Although international in outlook, the Editor especially encourages papers from the Asia Pacific. The journal also aims to provide clinicians, scientists and students of dentistry with a knowledge transfer platform for rapid publication of reports through an international journal, which will be available free online until 2012. Its scope, therefore, is broad, inclusive and international, but with a particular focus on Asia Pacific. The Editor welcomes manuscripts in the following key thematic areas in oral and maxillofacial sciences: Community Dentistry and Oral Epidemiology, Conservative Dentistry, Dental Biomaterials, Dental Pedagogy, Endodontics and Traumatology, Implant Dentistry, Oral Biosciences, Oral and Maxillofacial Surgery, Oral Medicine, Oral Pathology and Oral Microbiology, Orthodontics, Oral Radiology, Oral Rehabilitation, Paedodontics, Periodontology and Periodontal Medicine.